Identification of microorganisms using MALDI–TOF MS

One of the possible usages of MALDI–TOF MS is identification of microorganisms. It is highly accurate and faster than other methods and suitable for a large area of microorganisms. But unfortunately cannot reliably differentiate some closely related species. Often, it is possible to detect pathogenic organisms and use their accurate determination for monitoring environment, food processing or clinical diagnostics. Data obtained from mass spectra are compared with library of microorganisms and result of this was agreement or disagreement of microorganisms with specific spectrum.

Fig. 1: Scheme of MALDI-TOF mass spectrometer Fig. 2: Formula DHB (2,5 - dihydroxybenzoic acid)

1. Guo L., Ye L., Zhao Q., Ma Y., Yang J., Luo Y.: Journal of thoracic disease, 6, 534 (2014).
2. Trevino M., Areses P., Penalver M. D., Cortizo S., Pardo F., del Molino M. L. P., Garcia-Riestra C., Hernandez M., Llovo J., Regueiro B. J.: Anaerobe, 18, 37 (2012).
3. Pinto L., Poeta P., Vieira S., Caleja C., Radhouani H., Carvalho C., Vieira-Pinto M., Themudo P., Torres C., Vitorino R., Domingues P., Igrejas G.: Journal of Proteomics, 73, 1535 (2010). 4. Schafer M. O., Genersch E., Funfhaus A., Poppinga L., Formella N., Bettin B., Karger A.: Veterinary Microbiology, 170, 291 (2014).
5. Wang W., Xi H., Huang M., Wang J., Fan M., Chen Y., Shao H., Li X.: Journal of thoracic disease, 6, 524 (2014).
6. Chalupova J., Raus M., Sedlarova M., Sebela M.: Biotechnology Advances, 32, 230 (2014).
7. DeMarco M. L., Ford B. A.: Clinics in Laboratory Medicine, 33, 611 (2013).
8. Thomson J. J.: Proceedings of the Royal Society, 1, 1 (1913).
9. Thomson J. J.: Science (New York, N.Y.), 37, 360 (1913).
10. W. A. F.: Phys. Review, 316 (1918).
11. Aston F. W.: 152 (1922).
12. van den Boom D., Wjst M., Everts R. E.: Methods in molecular biology (Clifton, N.J.), 1015, 71 (2013).
13. Cho Y. T., Su H., Huang T. L., Chen H. C., Wu W. J., Wu P. C., Wu D. C., Shiea J.: Clinica Chimica Acta, 415, 266 (2013).
14. Samb-Ba B., Mazenot C., Gassama-Sow A., Dubourg G., Richet H., Hugon P., Lagier J. C., Raoult D., Fenollar F.: Plos One, 9, (2014).
15. Braga P. A. C., Tata A., dos Santos V. G., Barreiro J. R., Schwab N. V., dos Santos M. V., Eberlin M. N., Ferreira C. R.: Rsc Advances, 3, 994 (2013).
16. Sauer S., Kliem M.: Nature Reviews Microbiology, 8, 74 (2010).
17. Machen A., Drake T., Wang Y. F.: Plos One, 9, (2014).

PDF