Magnetic micro and nanoparticles for unique sequences barcoding

Kristyna Cihalova, Dagmar Chudobova, Pavel Kopel, Marie Konecna, Vojtech Adam, Rene Kizek

This review is focused on identification of bacterial species by their antigenes by barcoding oligonucleotides by application of magnetic micro and nanoparticles. In the first part of this review is discussed the structures and types of magnetic particles and their synthesis. In the next and the most important part we described the principle and the existing status of barcoding system and antigenes markers for identification of organisms such as animals, plants and microorganisms.

merlos-1
Fig. 1: Isolation of antigen using antibodies, oligonucleotides and magnetic separation followed by detection of binding of the oligonucleotide to the complementary odseparovaného olligonukleotidům using various methods.
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Fig. 2: Isolation of an oligonucleotide using complementary oligonucleotides to the magnetic particles and on the particle barkódovými with oligonucleotides complementary to the oligonucleotide at the same time in setting. Magnetic separation with subsequent detection of binding of the oligonucleotide to the complementary odseparovaného olligonukleotidům using various methods.

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