Není hanbou nic nevědět, ale je hanbou, nechtít se ničemu učit.
About the Cover:
Graphic art depicting the fluorescence labeling and detection of small RNA in the pathogenic bacteria Yersinia pestis. Artwork created by Leslie Sandoval, Los Alamos National Laboratory. View the article.
Here, we present a modification to single-molecule fluorescence in situ hybridization that enables quantitative detection and analysis of small RNA (sRNA) expressed in bacteria. We show that short ( 200 nucleotide) nucleic acid targets can be detected when the background of unbound singly dye-labeled DNA oligomers is reduced through hybridization with a set of complementary DNA oligomers labeled with a fluorescence quencher. By neutralizing the fluorescence from unbound probes, we were able to significantly reduce the number of false positives, allowing for accurate quantification of sRNA levels. Exploiting an automated, mutli-color wide-field microscope and data analysis package, we analyzed the statistics of sRNA expression in thousands of individual bacteria. We found that only a small fraction of either Yersinia pseudotuberculosis or Yersinia pestis bacteria express the small RNAs YSR35 or YSP8, with the copy number typically between 0 and 10 transcripts. The numbers of these RNA are both increased (by a factor of 2.5× for YSR35 and 3.5× for YSP8) upon a temperature shift from 25 to 37 °C, suggesting they play a role in pathogenesis. The copy number distribution of sRNAs from bacteria-to-bacteria are well-fit with a bursting model of gene transcription. The ability to directly quantify expression level changes of sRNA in single cells as a function of external stimuli provides key information on the role of sRNA in cellular regulatory networks.
Je otevřen přístup do database časopisů ACS z MENDELU přístupového bodu. Využijte tyto přístupy maximální míře.
http://pubs.acs.org/
V rámci řešení projektu MENDELU RESEARCH LIBRARY http://mereli.mendelu.cz/ byl na webových stránkách http://www.mendelu.cz/cz/sluzby_sz/icuk/databaze
zpřístupněn nový elektronický informační zdroj a to Wiley Current Protocols
http://onlinelibrary.wiley.com/browse/publications?type=labprotocols. Jedná se o trvalý nákup Current Protocols publikovaných 2013-2017.
dovoluji si Vás upozornit, že některé z knih, které jsme se rozhodli zakoupit z projektu SCI-INFO, jsou již k dispozici. Naleznete je v databázi EBSCO (http://search.ebscohost.com) v oddělení EBSCO eBooks - VUT eBooks pro STM. Knihy jsou vyhledatelné i z normálního rozhraní EBSCO.
Z Vámi požadovaných knihy byly zatím zakoupeny tyto:
531549 Modeling and Prediction of Polymer Nanocomposite Properties 2013 TECHNOLOGY & ENGINEERING / Materials Science
565781 In-situ Small-angle X-ray Scattering Investigation of Transient Nanostructure of Multi-phase Polymer Materials Under Mechanical Deformation 2013 MEDICAL / Family & General Practice
254721 Light, Water, Hydrogen: The Solar Generation of Hydrogen By Water Photoelectronics 2008 TECHNOLOGY & ENGINEERING / Power Resources / General
513752 Biomedical Applications of Mesoporous Ceramics: Drug Delivery, Smart Materials, and Bone Tissue Engineering 2013 MEDICAL / Instruments & Supplies
174656 Nonlinear and Adaptive Control : Tools and Algorithms for the User 2006 TECHNOLOGY & ENGINEERING / Robotics, TECHNOLOGY & ENGINEERING /
490396 Handbook of Nanoscopy 2012 SCIENCE / Microscopes & Microscopy
535247 Encyclopedia of Laser Research 2012 TECHNOLOGY & ENGINEERING / Lasers & Photonics
371167 Coherent Light Microscopy, Imaging and Quantitative Phase Analysis 2011 SCIENCE / Microscopes & Microscopy
V pátek 1. února za první tři premiérové vysílací hodiny (20.00–23.00 hod.) dosáhl Prima ZOOM v CS D15+ podílu na trhu 6,49 % (sledovanost 224 tisíc diváků). Celodenní podíl pak činil i přes znevýhodnění pozdního začátku 2,50 % share. Podle dostupných informací tak jde i přes výše uvedené časové znevýhodnění o nejsebevědomější start nového kanálu od počátku digitalizace. Nejsledovanějším pořadem byl v pátek v této CS dokument Bizarní obyvatelé moří (21.00–21.50 hod.). Ujít si ho nenechalo 286 tisíc diváků (7,74 % share).Že se v pátek večer nejednalo pouze o úvodní náhodný výsledek, potvrdila Prima ZOOM hned v sobotu, kdy přes velkou konkurenci sportovního přenosu v CS D15+ vystoupal celodenní podíl na trhu na 4,93 % share. Nejsledovanějším sobotním pořadem byl v této cílové skupině dokument Krokodýlí král, který si po desáté večer pustilo 204 tis. diváků (6,61 % share).A do třetice všeho dobrého se nový kanál výrazně prosadil i v neděli 3. února, kdy v CS D15+ dosáhl jeho celodenní podíl 4,65 % share. Tentokrát byl nejsledovanějším pořadem historický dokument Tajemná smrt Kleopatry, vysílaný po druhé hodině odpolední. Sledovalo ho 194 tis. diváků (7,72 % share). O mnoho pozadu nezůstal ani primetimeový dokument Vláda dinosaurů (20.00–20.43 hod.) se 187 tisíci diváků (4,30 % share) a odpolední Hitlerova tajná stíhačka (17.00–17.50 hod) se 182 tisíci diváků (6,33 % share).Zájem o televizní kanál se projevil i na stránkách prima-zoom.cz, kde během víkendu stoupl počet reálných uživatelů (RUest) na přibližně dvojnásobek (16 632 tisíc v neděli 3. února) a počet zobrazených stránek se přiblížil stu tisícům denně (95 928 tisíc v neděli 3. února). Skvěle se daří také facebookové stránce kanálu, která má nyní více než 24 tisíc fanoušků.
Title::The possible separation of 12-O-tetradecanoylphorbol-13-acetate-induced skin inflammation and hyperplasia by compound A
Author(s): : Kowalczyk, Piotr; Kowalczyk, Magdalena C.; Junco, Jacob J.; et al.
Source: MOLECULAR CARCINOGENESIS Volume: 52 Issue: 6 Pages: 488-496 DOI: 10.1002/mc.21883 Published: JUN 2013
Abstract: Activated glucocorticoid receptor (GR) acts via two different mechanisms: transcriptional regulation that requires DNA-binding, and proteinprotein interaction between GR and other transcription factors, such as nuclear factor kappa B (NF-B) or activator protein 1 (AP-1). It has been postulated that many important effects of glucocorticoids, including their anti-inflammatory properties, depend on GR's transrepressive effects on NF-B and AP-1. In the present study, we have employed a TPA-induced model of skin inflammation and epidermal hyperplasia to determine whether partial activation of the glucocorticoid receptor by compound A (CpdA) is sufficient to reverse the effect of TPA treatment. CpdA is a nonsteroidal GR modulator with high binding affinity, is capable of partial activation of GR. Topical application of TPA twice per week for 2wk results in inflammation and epidermal hyperplasia. TPA treatment also elevates levels of c-jun (AP-1 component), cyclooxygenase-2 (COX-2), p50 (NF-B component), interleukin-6 (IL-6), and tumor necrosis factor (TNF) in the skin. Fluocinolone acetonide (FA) (a full GR agonist) was able to completely reverse the above effects of TPA. When applied alone, CpdA increased the epidermal thickness and keratinocyte proliferation as well as levels of c-jun, COX-2, IL-6, and IFN-. However, CpdA treatment resulted in a decrease in the number of p50 positive cells induced by TPA, suggesting its role in inhibition of NF-B. The level of metallothionein-1 mRNA, regulated by GR was also significantly decreased in skin samples treated with CpdA. Our results suggest that CpdA is able to inhibit GR transactivation and activate only some transrepression properties of GR. (c) 2012 Wiley Periodicals, Inc.
Title:Tissue-specificity and phylogenetics of Pl-MT mRNA during Paracentrotus lividus embryogenesis
Author(s): :Russo, Roberta; Zito, Francesca; Matranga, Valeria
Source::GENE Volume: 519 Issue: 2 Pages: 305-310 DOI: 10.1016/j.gene.2013.01.063 Published: MAY 1 2013
Abstract: Metallothioneins (MTs) constitute a family of cysteine-rich, low molecular weight proteins, which generally provide protection against metal toxicity and oxidative stress counteracting the cell damage caused by essential and non-essential heavy metals. Equally important is the physiological role of MTs in the homeostasis of essential metals, which are involved in a wide variety of cellular processes. The aim of this work was to investigate the expression and the territorial localization of Paracentrotus lividus MT (Pl-MT) mRNA during sea urchin development by Quantitative Polymerase Chain Reaction (QPCR) and Whole Mount In Situ Hybridization (WMISH), as well as the phylogenetic comparison with selected MT homologs present in different phyla. We found that Pl-MT mRNA is accumulated in unfertilized eggs and constitutively expressed during development, with very low levels of maternal mRNA at cleavage stages, followed by a significant rise during gastrulation with a peak at the prism stage. Pl-MT mRNA was expressed in the vegetative plate at mesenchyme blastula, later restricted to the endoderm of gastrula embryos and finally to the gut of plutei. Indirect immunofluorescence (IF) using a specific antibody for the endoderm marker Endo1 demonstrated a co-localization with the Pl-MT transcripts in the midgut and hindgut after the intestine differentiation occurs and when larval feeding begins. Our results show for the first time the constitutive temporal and tissue-specific expression of MT in P. lividus embryos, providing new information for studies on the mechanisms controlling basal and induced MT gene expression. The analysis of the phylogenetic relationship of Pl-MT with homologs from different phyla, ranging from yeast to vertebrates, suggests the evolutionary process of these proteins, which could have been selected not only on the basis of their ability to bind metals but also by their tissue-specificity. (C) 2013 Elsevier B.V. All rights reserved.
Title:Microarray analysis of the inflammatory and immune responses in head kidney turbot leucocytes treated with resveratrol
Author(s): : Dominguez, Berta; Pardo, Belen G.; Noia, Manuel; et al..
Source::INTERNATIONAL IMMUNOPHARMACOLOGY Volume: 15 Issue: 3 Pages: 588-596 DOI: 10.1016/j.intimp.2013.01.024 Published: MAR
Abstract: A DNA oligo-microarray enriched in genes and involved in inflammatory and immune responses was used to evaluate the effects of resveratrol on gene expression in turbot head kidney leucocytes. Leucocytes were cultured for 3, 6 and 24 h, in the presence or absence of resveratrol, or were stimulated with the membrane fraction of the parasite Philasterides dicentrarchi or with the membrane plus resveratrol. Gene expression changed considerably in control cells, and several of the regulated genes were related to inflammatory and immune responses and to the cytoskeleton. Similar changes in gene expression occurred in control cells and in cells stimulated with P. dicentrarchi membrane fraction. Treatment with resveratrol induced changes in the expression (mostly down-regulation) of several genes involved in immune responses and inflammation. Thus, the down-regulation of the transcription factor PU.1, pentraxin-multidomain protein, heme oxygenase I, S100 calcium-binding protein A-16 (S100A16) and the signal transducer and activator of transcription 4 was observed after all three incubation times. The down-regulation of the suppressor of cytokine signalling 3a, LPS-induced tumour necrosis alpha, hepcidin, metallothionein, TLR8 and the calcium dependent lectin A was observed after 3 and 6 h. Resveratrol also decreased the expression of CCL20, IL-8, apolipoprotein E and glutathione 5-transferase after incubation for 6 and 24 h, and of TNF-alpha after incubation for 3 and 24 h. Resveratrol also induced strong regulation of several cytoskeleton-related genes. The use of the turbot oligo-microarray enabled us to discover genes whose expression was not previously suspected of being modulated by this polyphenol. (C) 2013 Elsevier B.V. All rights reserved.
Journal | Title | Deadline | internet |
Int. J. Mol. Sci. | Redox Signaling in Biology and Patho-Biology | 30.12.2013 | http://www.mdpi.com/journal/ijms/special_issues/redox_signaling |
Int. J. Mol. Sci | Oxidative Stress and Ageing | 20.5.2013 | http://www.mdpi.com/journal/ijms/special_issues/oxidative_stress_aging |
Int. J. Mol. Sci. | Oxidative Stress in Cardiovascular Disease | 30.5.2013 | http://www.mdpi.com/journal/ijms/special_issues/cardiovascular-disease |
Int. J. Mol. Sci. | Magnetic Nanoparticles 2013 | 15.6.2013 | http://www.mdpi.com/journal/ijms/special_issues/magnetic-nanoparticles_2013 |
Int. J. Mol. Sci. | Advances in Cancer Diagnosis | 30.4.2013 | http://www.mdpi.com/journal/ijms/special_issues/cancer-diagnosis |
Int. J. Mol. Sci. | Molecular Research in Urology | 20.4.2013 | http://www.mdpi.com/journal/ijms/special_issues/mol_res_uro |
Int. J. Env. Res. Publ. Health | Antimicrobial Resistance Prevention and Control | 31.6.2013 | http://www.mdpi.com/journal/ijerph/special_issues/antimicrobial-resistance |
Water | "Analytical Chemistry of Water" | 31.5.2013 | http://www.mdpi.com/journal/water/special_issues/analytical-chemistry |
Sensors | Aptasensors | 31.10.2013 | http://www.mdpi.com/journal/sensors/special_issues/Aptasensors |
Sensors | Ultramicroelectrode Electrochemistry | 31.8.2013 | http://www.mdpi.com/journal/sensors/special_issues/ultramicroelectrode |
Sensors | Nanotube and Nanowire Sensors | 31.5.2013 | http://www.mdpi.com/journal/sensors/special_issues/nanotube-nanowire-sensors |
Int. J.Molecular Sci. | Molecular Research of Carcinogenesis | 30.6.2013 | http://www.mdpi.com/journal/ijms/special_issues/molecular_research_of_carcinogenesis |
Toxins | Bacterial Toxins and Carcinogenesis | 30.4.2013 | http://www.mdpi.com/journal/toxins/special_issues/toxin-carcinog |
Electrophoresis | Miniaturization 2013 | 15.4.2013 | http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1522-2683 |
Molecules | Synthesis of Nucleosides, Nucleotides and Their Derivatives | 31.8.2013 | http://www.mdpi.com/journal/molecules/special_issues/synthesis-nucleosides-derivatives |
Molecules | G-Quadruplexes & i-Motif DNA | 31.8.2013 | http://www.mdpi.com/journal/molecules/special_issues/G-quadruplexes_i-motif_DNA |
Int. J. Mol. Sci. | Redox Signaling in Biology and Patho-Biology | 30.12.2013 | http://www.mdpi.com/journal/ijms/special_issues/redox_signaling |
Int. J. Mol. Sci. | Molecular Bases of Cancer Research | 30.8.2013 | http://www.mdpi.com/journal/ijms/special_issues/bases_of_cancer_research |
Int. J. Mol. Sci. | Bioactive Nanoparticles 2013 | 15.10.2013 | http://www.mdpi.com/journal/ijms/special_issues/bioactive_nanoparticles_2013 |
Materials | Carbon Nanotubes | 30.4.2013 | http://www.mdpi.com/journal/materials/special_issues/c_nanotubes |
Curr. Pept.Sci. | Peptides | 30.5.2013 | |
Electrophoresis | innovative approach to the use CE | 30.5.2013 | |
acta biochemica polonica | speciálni výživa | 30.12.2013 |
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