Technical concept of 3D printed fluidic biosensor with polydimethylsiloxane chip based on fluorescence detection system

A biosensor is an analytical device, which can be easily employed for detection of broad spectra of analytes, including molecules, bacteria, viruses, etc. [1]. The development of biosensors provides an accurate, sensitive and specific detection, together with portability and the ability to furnish continuous real time signals of analytes levels in various matrixes. According to the definition, firmly established in the analytical field “biosensor” is a detection system that relies on a biomolecule for molecular recognition and a transducer to produce an observable output [2]. Molecular recognition element (e. g. antibodies, nucleic acids, receptor proteins, whole cells or bacteria) is fundamental component that interacts with the studied analyte [3]. Currently, very promising recognition elements are produced as a result of driven recombination in biological engineering. The transducers can work in different ways, such as physicochemical, optical, piezoelectric, electrochemical, etc.). Their role is the transformation of obtained signal, which goes from the analyte-recognition element interaction [4]. The transformation leads to more easily measured and quantified signals. Nowadays, few commonly used biosensors such as glucose biosensor are in every-day use all over the world. The development of novel biosensors for easy, fast and cheap detection of various types of biomarkers for cancer, oxidative stress or metabolical disorders, can significantly enhance the treatment successes and the survival rates. From these reasons, we suggested a technical concept of 3D printed biosensor with polydimethylsiloxane (PDMS) chip, utilizing the fluorescence detection, which is one of the most sensitive, to serve as a universal platform for detection of various types of biomarkers. Proposed biosensor was particularly designed to work in coupling with quantum dots-based labeling, which offers exceptional quantum yields. Various recognition biomolecules can be easily labeled by quantum dots, thus high specifity and boosted sensitivity can be achieved.

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