Secreted trimeric viral envelope proteins as a tool for new vaccine design and biochemical assays

Vladimir Pekarik

Varies viral envelope proteins are formed by trimeric modules that are highly stable while anchored through the transmembrane domain in the virus membrane. Unfortunately, to raise vaccines against the whole viruses could lead to the production of antibodies against hijacked cellular proteins that can cause adverse effects. Therefore, pure protein preparations are used for vaccines production. It was found that the use of monomeric immunogens is insufficient in induction of neutralising antibodies. Protective antibodies were more often produced when trimeric ENV proteins were used. Here, we describe technical aspects of ENV proteins processing in host cells, the way to construct stable trimeric immunogens, and recent progress with construction of soluble trimers of ENV from Ebola, HIV, rabies, and influenza viruses.

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