Monitoring of metallothionein levels in biological organism exposed to the metal elements and compounds

In this study, the basic facts about the relationship between metallothionein and metals are summarized, determining the affinity of the interaction of metallothionein with different metals and the importance of this interaction for the functioning of organisms. In two described experiments there was presented significance of metallothionein determination in organism exposure to heavy metals.

Fig. 1: Structure of metallothionein

Fig. 2: Detailed description of the apparatus used for the electrochemical detection of metallothionein

1. Kagi, J.H.R.; Schaffer, A. Biochemistry of metallothionein. Biochemistry. 1988, 27, 8509-8515.
2. Bamber, B.A.; Masters, B.A.; Hoyle, G.W.; Brinster, R.L.; Palmiter, R.D. Leukemia inhibitory factor induces neurotransmitter switching in transgenic mice. Proceedings of the National Academy of Sciences of the United States of America. 1994, 91, 7839-7843.
3. Cobbett, C.; Goldsbrough, P. Phytochelatins and metallothioneins: Roles in heavy metal detoxification and homeostasis, 2002.
4. Nath, R.; Kambadur, R.; Gulati, S.; Paliwal, V.K.; Sharma, M. Molecular aspects, physiological-function, and clinical-significance of metallothioneins Crc Critical Reviews in Food Science and Nutrition. 1988, 27, 41-85. 5. Simpkins, C.O. Metallothionein in human disease. Cellular and Molecular Biology. 2000, 46, 465-488.
6. Ghoshal, K.; Jacob, S.T. Regulation of metallothionein gene expression. Progress in Nucleic Acid Research and Molecular Biology, Vol 66. 2001, 66, 357-384.
7. Hamer, D.H. Metallothionein - an overview. Marine Environmental Research. 1988, 24, 171-171.
8. Masters, B.A.; Quaife, C.J.; Erickson, J.C.; Kelly, E.J.; Froelick, G.J.; Zambrowicz, B.P.; Brinster, R.L.; Palmiter, R.D. Metallothionein-III is expressed in neurons that sequester zinc in synaptic vesicles. Journal of Neuroscience. 1994, 14, 5844-5857.
9. Moffatt, P.; Seguin, C. Expression of the gene encoding metallothionein-3 in organs of the reproductive system. DNA and Cell Biology. 1998, 17, 501-510.
10. Zeng, J.; Heuchel, R.; Schaffner, W.; Kagi, J.H.R. Metallothionein (apometallothionein) can modulate DNA-binding and transcription activation by zinc finger containing factor-SP1. Febs Letters. 1991, 279, 310-312.
11. Barnas, C.; MartelPlanche, G.; Furukawa, Y.; Hollstein, M.; Montesano, R.; Hainaut, P. Inactivation of the p53 protein in cell lines derived from human esophageal cancers. International Journal of Cancer. 1997, 71, 79-87.
12. Studer, R.; Vogt, C.P.; Cavigelli, M.; Hunziker, P.E.; Kagi, J.H.R. Metallothionein accretion in human hepatic cells is linked to cellular proliferation. Biochemical Journal. 1997, 328, 63-67.
13. Davis, S.R.; Cousins, R.J. Metallothionein expression in animals: A physiological perspective on function (Reprinted from vol 130, pg 1085, 2000). Journal of Nutrition. 2002, 132, 1085-1088.
14. Roesijadi, G.; Bogumil, R.; Vasak, M.; Kagi, J.H.R. Modulation of DNA binding of a tramtrack zinc finger peptide by the metallothionein-thionein conjugate pair. Journal of Biological Chemistry. 1998, 273, 17425-17432.
15. Sato, M.; Bremner, I. Oxygen free-radicals and metallothionein Free Radical Biology and Medicine. 1993, 14, 325-337.
16. Theocharis, S.E.; Margeli, A.P.; Klijanienko, J.T.; Kouraklis, G.P. Metallothionein expression in human neoplasia. Histopathology. 2004, 45, 103-118.
17. Petrlova, J.; Potesil, D.; Mikelova, R.; Blastik, O.; Adam, V.; Trnkova, L.; Jelen, F.; Prusa, R.; Kukacka, J.; Kizek, R. Attomole voltammetric determination of metallothionein. Electrochimica Acta. 2006, 51, 5112-5119.
18. Raspor, B.; Paic, M.; Erk, M. Analysis of metallothioneins by the modified Brdicka procedure. Talanta. 2001, 55, 109-115.
19. Waalkes, M.P.; Harvey, M.J.; Klaassen, C.D. Relative invitro affinity of hepatic metallothionein for metals. Toxicology Letters. 1984, 20, 33-39.

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