League against cancer in Laboratory of Metallomics and Nanotechnologies

Program of WCD 2015 was dedicated to ten researchers from the Laboratory of metallomics and nanotechnologies and their lectures (Fig. 1). The whole series of lectures were introduced by information of the League against cancer Prague activities and regular actions also organized under its auspices on the Laboratory of metalomics and nanotechnology ground. For interest was introduced the cooperation of both subjects which has been continued on the project level since 2006. The connection of some viruses and tumor diseases has been not fully understood yet, so the various relations between malignant progression and presence of SV40 (simian vacuolating virus 40) viral DNA, distributed between 1955 and 1963 in poliomyelitis vaccine as a contamination, were discussed. The ability of virus interference with the function of tumor suppressor p53 was described in laboratory animal tests; however the connections in human have not been clearly described yet.

1. Kaluzna-Czaplinska, J.; Jozwik, J. Current applications of chromatographic methods for diagnosis and identification of potential biomarkers in cancer. Trac-Trends Anal. Chem. 2014, 56, 1-12.
2. Yang, Z.Y.; Sweedler, J.V. Application of capillary electrophoresis for the early diagnosis of cancer. Anal. Bioanal. Chem. 2014, 406, 4013-4031.
3. Gao, M.X.; Qi, D.W.; Zhang, P.; Deng, C.H.; Zhang, X.M. Development of multidimensional liquid chromatography and application in proteomic analysis. Expert Rev. Proteomics 2010, 7, 665-678.
4. Kolch, W.; Neususs, C.; Peizing, M.; Mischak, H. Capillary electrophoresis - mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery. Mass Spectrom. Rev. 2005, 24, 959-977.
5. Issaq, H.J.; Veenstra, T.D. Two-dimensional polyacrylamide gel electrophoresis (2d-page): Advances and perspectives. Biotechniques 2008, 44, 697-700.
6. Yoo, C.; Pal, M.; Miller, F.R.; Barder, T.J.; Huber, C.; Lubman, D.M. Toward high sequence coverage of proteins in human breast cancer cells using on-line monolith-based hplc-esi-tof ms compared to ce ms. Electrophoresis 2006, 27, 2126-2138.
7. Chen, Y.R.; Ma, Z.H.; Li, A.Y.; Li, H.W.; Wang, B.; Zhong, J.; Min, L.S.; Dai, L.C. Metabolomic profiling of human serum in lung cancer patients using liquid chromatography/hybrid quadrupole time-of-flight mass spectrometry and gas chromatography/mass spectrometry. J. Cancer Res. Clin. Oncol. 2015, 141, 705-718.
8. Musharraf, S.G.; Mazhar, S.; Choudhary, M.I.; Rizi, N.; Atta ur, R. Plasma metabolite profiling and chemometric analyses of lung cancer along with three controls through gas chromatography-mass spectrometry. Sci Rep 2015, 5.
9. Ma, W.; Gao, P.; Fan, J.; Hashi, Y.; Chen, Z.L. Determination of breath gas composition of lung cancer patients using gas chromatography/mass spectrometry with monolithic material sorptive extraction. Biomed. Chromatogr. 2015, 29, 961-965.
10. Ma, H.Y.; Li, X.; Chen, J.M.; Wang, H.J.; Cheng, T.T.; Chen, K.; Xu, S.F. Analysis of human breath samples of lung cancer patients and healthy controls with solid-phase microextraction (spme) and flow-modulated comprehensive two-dimensional gas chromatography (gc x gc). Anal. Methods 2014, 6, 6841-6849.
11. Premstaller, A.; Oberacher, H.; Walcher, W.; Timperio, A.M.; Zolla, L.; Chervet, J.P.; Cavusoglu, N.; van Dorsselaer, A.; Huber, C.G. High-performance liquid chromatography-electrospray ionization mass spectrometry using monolithic capillary columns for proteomic studies. Anal. Chem. 2001, 73, 2390-2396.
12. Qian, W.J.; Jacobs, J.M.; Liu, T.; Camp, D.G.; Smith, R.D. Advances and challenges in liquid chromatography-mass spectrometry-based proteomics profiling for clinical applications. Mol. Cell. Proteomics 2006, 5, 1727-1744.
13. Zhao, Y.Y.; Cheng, X.L.; Vaziri, N.D.; Liu, S.M.; Lin, R.C. Uplc-based metabonomic applications for discovering biomarkers of diseases in clinical chemistry. Clin. Biochem. 2014, 47, 16-26.
14. Dakna, M.; He, Z.Y.; Yu, W.C.; Mischak, H.; Kolch, W. Technical, bioinformatical and statistical aspects of liquid chromatography-mass spectrometry (lc-ms) and capillary electrophoresis-mass spectrometry (ce-ms) based clinical proteomics: A critical assessment. J. Chromatogr. B 2009, 877, 1250-1258.
15. Zhang, X.W.; Wei, D.; Yap, Y.; Li, L.; Guo, S.Y.; Chen, F. Mass spectrometry-based „omics“ technologies in cancer diagnostics. Mass Spectrom. Rev. 2007, 26, 403-431.

PDF