logo ban ban logo

Učit se znamená objevovat to, co už víš.
Konat znamená demonstrovat, že to to víš.
Učit druhé znamená připomínat jim, že to vědí stejně dobře jako ty.
Všichni jste zároveň žáci, praktikanti a učitelé.

Richard Bach

mendel

Výzkum

Electrochemical characterization of pna oligonucleotide of neuraminidase gene

Combining efforts of biochemist Peter Nielsen’s and organic chemist Ole Buchardt’s groups led to the investigation of peptide nucleic acid during the 1990s. It was suggested that ?-helical poly-?-benzyglutamate (PBG) with alternating nucleobases and acridine moieties could bind sequences selectively to duplex DNA by combining Hoogsteen base pair formation (the type of base pairing that occurs between adenine and thymine where adenine N7 acts as the hydrogen bonding acceptor rather than N1 of the Watson-Crick geometry) and intercalation with the helical backbone in the major groove because aromatic chromophores form stacked complexes with PBG. The proposed peptide nucleobase compound was named peptide nucleic acid (PNA) [1,2]. Up to now, there have been published more than 130 000 articles about PNA. Application of PNA can be divided into four main categories, namely PNA as biosensor [3-5], PNA as a probe for diagnosis and detection [6-13], PNA as a tool for functional genomics and molecular biology [14-20], and PNA for antigene and antisense therapy [21-28].

PNA mimics DNA, in which the entire deoxyribose-phosphate backbone is replaced with a structurally homomorphous uncharged, achiral polyamide backbone composed of N-(2-aminoethyl)glycine units linked by peptide bonds (Fig. 1A). The different bases (purines and pyrimidines) are joined to the backbone by methylene carbonyl linkages [29], which is shown in Fig. 1A. Neutral property of peptide backbone eliminates electrostatic repulsion between hybridized strands. Some other properties of PNA are proper interbase distances, rigid amino bonds, and flexible aminoethyl linkers and finally intermolecular hydrogen bonding, which can increase affinity of binding between PNA and complementary DNA in comparison with binding of DNA with DNA [30]. Common methods for electrochemical detection of PNA are square wave voltammetry (SWV), cyclic voltammetry (CV) [31], and differential pulse voltammetry (DPV) in different buffer such as Tris-HCl containing NaCl [5,32] and/or phosphate buffer [33]. In this study, new method for electrochemical detection of PNA was investigated. DPV and DPV coupled with adsorptive transfer technique (AdT DPV) were employed for monitoring Brdicka reaction of PNA. The effect of accumulation time, temperature of Brdicka electrolyte, and concentration of PNA on electrochemical behaviour of PNA was described here.

Práce je spojená s projektem NanoBioMetalNet CZ.1.07/2.4.00/31.0023

Zemědělská 1/1665
613 00 Brno
Budova D		Tel.: +420 545 133 350
Fax.: +420 545 212 044