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Konat znamená demonstrovat, že to to víš.
Učit druhé znamená připomínat jim, že to vědí stejně dobře jako ty.
Všichni jste zároveň žáci, praktikanti a učitelé.

Richard Bach

Tisková zpráva
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mendel
mendel

2015

Zprávy z laboratoře / Laboratory information

liposon liposon

Picture of the week

Pravidelný seminář (29 T) Your barometer is just as accurate a weather forecaster as the TV meteorologist you watch with the news. Under most conditions it forecasts weather for the 12 to 24 hours ahead. Its mission is to measure air or atmospheric pressure and indicate its rising or falling. The pressure is shown on your barometer's dial, usually expressed in "inches" referring to "inches of mercury" (inch Hg). The early barometers measured the pressure by the rise or fall of a column of mercury. This mercury barometer was invented back in 1643 by a pupil of Galileo named Evangelista Toricelli. Meteorologists use "millibars" in charting atmospheric pressure and your barometer has a second scale or ring which reads in millibars (mb), or "hectopascals" (hPa). The latter is used in some countries as a unit of pressure. Standard air pressure at standard elevation (sea level) at 15°C and 45° latitude is 1013 hPa or 29.92 inches of mercury.

LABORATOŘ METALOMIKY A NANOTECHNOLOGIÍ Výzva k výběrovému řízení na pozice vědeckých pracovníků

Call 1
• Fyzikální chemie a elektrochemie
Call 2
• Kapilární a gelová elektroforéza
Call 3
• Molekulární biologie, mikrobiologie a virologie

Termín pro podání přihlášek: 1. 1. 2015 do 25. 1. 2015
Kvalifikace: Ph.D. a více
Nástup: IHNED

LABORATOŘ METALOMIKY A NANOTECHNOLOGIÍ Výzva k mimořádnému výběrovému řízení na pozice postgraduálního studia

Call 1
• Molekulární biologie viru EBOLA – metody pro správný odběr vzorku, skladování a transport
Call 2
• Molekulární biologie viru EBOLA – metody izolace proteinů a nukleových kyselin viru nanočásticemi
Call 3
• Vývoj elektrochemického detekčního systému s elektrochemickou detekcí pro online analýzu těžkých kovů
Call 4
• Vývoj metody využívající mikroskop s mikroelektrodami pro studium živých buněk

Termín pro podání přihlášek: 1. 1. 2015 do 25. 1. 2015
Počátek studia: 2.2.2015

BBC News interview in our laboratory.

http://www.dw.de/top-stories/sci-tech/s-12526

The center for nanotechnology collaboration. Extend your research with new partners & Collaborate with the world's best lab

My name is Pierre Gobet Lalanne and I am part of the french start-up NanoThinking, which has released NanoTechValley last week and I am writing to you to introduce it. NanoTechValley is a website which will enable you to present and share scientific projects with a community of researchers with an interest in nanotechnology in order to develop new collaborations. www.nanotechnology-collaboration.com

Laboratory research

Pravidelný seminář (29 T)Embryology is the study of the sequential development of various tissues, organs and systems from conception to birth. The observation and description of different embryo species development helps us to understand the products of genetic and environmental interaction. Genetic manipulation, induction of drugs, exposure to disease, radioactive tagging or dyeing of developing tissues and x-ray treatments are some of the commonly performed experiments that provide information that can be applied to human development and birth defects [1].The studies on chicken embryos represent the transition between the in vitro studies on cell cultures and in vivo experiments on mammalian organisms. The experiments on mammalian animal models are costly and generate ethical issue. Using of non-mammalian vertebrate embryos is possible without any licence and is reproducible with high throughput. The experiments on embryos are possible till they are able of independent feeding, it means the hole developmental period (21 days) of chicken embryos [2]. Chicken embryos are often studied because of the easy access through the shell and their rapid development [3]. The advantageous procedure for chicken embryo observation is x-ray method in combination with fluorescence measurement of the labeled tissues [4]. One of the most frequently used optical labeling agents are quantum dots (QDs) [5].

Journal of Metallomics and Nanotechnologies

Pravidelný seminář (29 T) Čtvrté číslo právě vyšlo
Časopis Journal of Metallomics and Nanotechnologies vychází pouze elektronicky, čtvrtletně. Jeho obsahové zaměření je v oblastí nano-biochemie, nanotechonologie, biomedicína a nanomedicína. Časopis vychází bez regionálních mutací v českém, slovenském a anglickém jazyce. Vydavatel: Laboratoř metalomiky a nanotechnologií Mendelova univerzita v Brně, Zemědělská 1, 613 00, Brno, Česká republika http://web2.mendelu.cz/af_239_nanotech/J_Met_Nano/index.html

Zprávy z výzkumu/ Scientific Reports



Implication of high risk Human papillomavirus HR-HPV infection in prostate cancer in Indian population- A pioneering case-control analysis
W3 Star-forming Region Prostate cancer is the second most common cancer with sexual history as a consistent risk factor. This is the pioneering study that evaluates the frequency of HPV infection in prostate cancer in India. Ninety five (95) histopathologically confirmed cancer and fifty five (55) BPH from Indian population were analyzed for HPV infection using a pair of consensus sequence primer followed by type specific PCRs for both high-risk and low-risk HPV types. The data demonstrate HPV infection in 41% of prostate tumor biopsies and 20% in BPH. Subsequent PCR- based HPV typing using type - specific primers revealed 32% were infected with HPV type 16 whereas 6% were found to be positive for HPV type 18, while in BPH controls only 5% of the BPH controls were infected with HPV 16 and this difference was highly significant (p = 0.0004). Significant proportion of HPV infected (74%) cases belonged to stage III and IV (p 0.001) with a high Gleason score ?8 (p = 0.003). The study represents for the first time the incidence of HPV infection in prostate cancer in Indian population and strengthens the hypothesis that HPV infection could be one of the co factor associated with progression of prostate cancer.
Genetic Diversity of Avian Influenza A (H10N8) Virus in Live Poultry Markets and Its Association with Human Infections in China
W3 Star-forming Region Following the first human infection with the influenza A (H10N8) virus in Nanchang, China in December 2013, we identified two additional patients on January 19 and February 9, 2014. The epidemiologic, clinical, and virological data from the patients and the environmental specimen collected from 23 local live poultry markets (LPMs) were analyzed. The three H10N8 cases had a history of poultry exposure and presented with high fever (>38°C), rapidly progressive pneumonia and lymphopenia. Substantial high levels of cytokines and chemokines were observed. The sequences from an isolate (A/Environment/Jiangxi/03489/2013 [H10N8]) in an epidemiologically linked LPM showed highly identity with human H10N8 virus, evidencing LPM as the source of human infection. The HA and NA of human and environmental H10N8 isolates showed high identity (99.1–99.9%) while six genotypes with internal genes derived from H9N2, H7N3 and H7N9 subtype viruses were detected in environmental H10N8 isolates. The genotype of the virus causing human infection, Jiangxi/346, possessed a whole internal gene set of the A/Environment/Jiangxi/10618/2014(H9N2)-like virus. Thus, our findings support the notion that LPMs can act as both a gene pool for the generation of novel reassortants and a source for human infection, and intensive surveillance and management should therefore be conducted.
Nanostructured Zr-Pd Metallic Glass Thin Film for Biochemical Applications
W3 Star-forming Region Zr-Pd metallic glassy thin films with a hierarchical nano-scale structure, produced by magnetron sputtering of the Zr and Pd powder mixture, demonstrate a unique combination of physical and biochemical properties. Thermal stability of the nano-structured glassy samples, their resistance to oxidation in dry air and phase transformation behavior are discussed in the present work. These binary alloy samples also show exceptionally high corrosion resistance and spontaneous passivation in a simulated body fluid. Experiments on the catalytic activity and biocompatibility of this nanostructured metallic glass indicate that this is a very suitable material for biochemical applications. Compared to the multicomponent alloys studied earlier this binary alloy has much simpler chemical composition, which makes preparation of the sample with defined stoichiometry easier, especially when the elements have different sputtering rates.
Poultry farms as a source of avian influenza A (H7N9) virus reassortment and human infection
W3 Star-forming Region Live poultry markets are a source of human infection with avian influenza A (H7N9) virus. On February 21, 2014, a poultry farmer infected with H7N9 virus was identified in Jilin, China, and H7N9 and H9N2 viruses were isolated from the patient's farm. Reassortment between these subtype viruses generated five genotypes, one of which caused the human infection. The date of H7N9 virus introduction to the farm is estimated to be between August 21, 2013 (95% confidence interval [CI] June 6, 2013- October 6, 2013) and September 25, 2013 (95% CI May 28, 2013-January 4, 2014), suggesting that the most likely source of virus introduction was the first batch of poultry purchased in August 2013. The reassortment event that led to the human virus may have occurred between January 2, 2014 (95% CI November 8, 2013-February 12, 2014) and February 12, 2014 (95% CI January 19, 2014-February 18, 2014). Our findings demonstrate that poultry farms could be a source of reassortment between H7N9 virus and H9N2 virus as well as human infection, which emphasizes the importance to public health of active avian influenza surveillance at poultry farms
Identification of molecular signature of head and neck cancer stem-like cells
W3 Star-forming Region Head and neck squamous cell carcinoma (HNSCC) is the sixth most prevalent cancer in the world. HNSCC remains difficult to treat, and despite advances in treatment, overall survival rate has modestly improved over the past several years. Poor survival rate is attributed to high frequency of local recurrence and distant metastasis. Cancer stem-like cells (CSCs) have been implicated in tumor recurrence and confer resistance to anti-cancer therapy treatment. In this study, we have characterized genes that are modulated in HNSCC-CSCs and can be targeted in future as potential therapeutics. CSCs were isolated from HNSCC cells (oralspheres) and examined for tumorigenicity in immunodeficient mice. We observed aggressive tumor growth with oralspheres as compared to parental cells. The CSC-derived tumors were grossly extremely vascularized and expressed VEGFR1. We next analyzed the molecular determinant of oralspheres. In addition to CD133 and Nanog, we observed significant higher expression of Notch1 protein in the oralspheres. There was differential expression of angiogenesis and invasive marker genes such as angiopoietin1, integrin ß3, MMP9 and THBS1. Interestingly, c-Fos was upregulated in oralspheres as compared to parental cells. Our observations suggest that understanding the molecular determinant of oralspheres will help in developing future therapeutic modalities against treatment resistant HNSCC.
3D-Printed Microfluidic Device for the Detection of Pathogenic Bacteria Using Size-based Separation in Helical Channel with Trapezoid Cross-Section
W3 Star-forming Region A facile method has been developed to detect pathogenic bacteria using magnetic nanoparticle clusters (MNCs) and a 3D-printed helical microchannel. Antibody-functionalized MNCs were used to capture E. coli (EC) bacteria in milk, and the free MNCs and MNC-EC complexes were separated from the milk using a permanent magnet. The free MNCs and MNC-EC complexes were dispersed in a buffer solution, then the solution was injected into a helical microchannel device with or without a sheath flow. The MNC-EC complexes were separated from the free MNCs via the Dean drag force and lift force, and the separation was facilitated in the presence of a sheath flow. The concentration of the E. coli bacteria was determined using a light absorption spectrometer, and the limit of detection was found to be 10 cfu/mL in buffer solution and 100 cfu/mL in milk.
Frosty Slopes on Mars
W3 Star-forming Region This image of an area on the surface of Mars, approximately 1.5 by 3 kilometers in size, shows frosted gullies on a south-facing slope within a crater. At this time of year, only south-facing slopes retain the frost, while the north-facing slopes have melted. Gullies are not the only active geologic process going on here. A small crater is visible at the bottom of the slope. The image was acquired on Nov. 30, 2014, by the High Resolution Imaging Science Experiment (HiRISE) camera, one of six instruments on NASA's Mars Reconnaissance Orbiter. The University of Arizona, Tucson, operates HiRISE, which was built by Ball Aerospace & Technologies Corp., Boulder, Colorado. NASA's Jet Propulsion Laboratory, a division of the California Institute of Technology in Pasadena, manages the Mars Reconnaissance Orbiter Project for NASA's Science Mission Directorate, Washington.
Blood biocompatibility of surface-bound multi-walled carbon nanotubes
W3 Star-forming RegionBlood clots when it contacts foreign surfaces following platelet activation. This can be catastrophic in clinical settings involving extracorporeal circulation such as during heart–lung bypass where blood is circulated in polyvinyl chloride tubing. Studies have shown, however, that surface-bound carbon nanotubes may prevent platelet activation, the initiator of thrombosis. We studied the blood biocompatibility of polyvinyl chloride, surface-modified with multi-walled carbon nanotubes in vitro and in vivo. Our results show that surface-bound multi-walled carbon nanotubes cause platelet activation in vitro and devastating thrombosis in an in vivo animal model of extracorporeal circulation. The mechanism of the pro-thrombotic effect likely involves direct multi-walled carbon nanotube-platelet interaction with Ca2 +-dependant platelet activation. These experiments provide evidence, for the first time, that modification of surfaces with nanomaterials modulates blood biocompatibility in extracorporeal circulation. From the Clinical Editor In this study, the blood biocompatibility of polyvinyl chloride, surface-modified with multi-walled carbon nanotubes was investigated both in vitro and in vivo, showing that surface-bound multi-walled carbon nanotubes cause platelet activation in vitro, and devastating thrombosis in an in vivo animal model of extracorporeal circulation.
Polysilsesquioxane nanoparticles for triggered release of cisplatin and effective cancer chemoradiotherapy
W3 Star-forming Region Chemoradiotherapy is a well-established treatment paradigm in oncology. There has been strong interest in identifying strategies to further improve its therapeutic index. An innovative strategy is to utilize nanoparticle (NP) chemotherapeutics in chemoradiation. Since the most commonly utilized chemotherapeutic with radiotherapy is cisplatin, the development of an NP cisplatin for chemoradiotherapy has the highest potential impact on this treatment. Here, we report the development of an NP comprised of polysilsesquioxane (PSQ) polymer crosslinked by a cisplatin prodrug (Cisplatin-PSQ) and its utilization in chemoradiotherapy using non-small cell lung cancer as a disease model. Cisplatin-PSQ NP has an exceptionally high loading of cisplatin. Cisplatin-PSQ NPs were evaluated in chemoradiotherapy in vitro and in vivo. They demonstrated significantly higher therapeutic efficacy when compared to cisplatin. These results suggest that the Cisplatin-PSQ NP holds potential for clinical translation in chemoradiotherapy. From the Clinical Editor These authors present an innovative strategy utilizing the presence of platinum in nanoparticle chemotherapeutics for chemoradiation, demonstrating its efficacy in a non-small cell lung cancer model.
Magnetic properties and antitumor effect of nanocomplexes of iron oxide and doxorubicin
W3 Star-forming Region We present a technology and magneto-mechanical milling chamber for the magneto-mechano-chemical synthesis (MMCS) of magneto-sensitive complex nanoparticles (MNC) comprising nanoparticles Fe3O4 and anticancer drug doxorubicin (DOXO). Magnetic properties of MNC were studied with vibrating magnetometer and electron paramagnetic resonance. Under the influence of mechano-chemical and MMCS, the complex show a hysteresis curve, which is typical for soft ferromagnetic materials. We also demonstrate that Lewis lung carcinoma had a hysteresis loop typical for a weak soft ferromagnet in contrast to surrounding tissues, which were diamagnetic. Combined action of constant magnetic field and radio frequency moderate inductive hyperthermia (RFH) below 40 °C and MNC was found to induce greater antitumor and antimetastatic effects as compared to conventional DOXO. Radiospectroscopy shows minimal activity of FeS-protein electron transport chain of mitochondria, and an increase in the content of non-heme iron complexes with nitric oxide in the tumor tissues under the influence of RFH and MNC. From the Clinical Editor This study reports on the top-down synthesis of magneto-sensitive complex nanoparticles comprised of Fe3O4 nanoparticles and doxorubicin. Authors also found that Lewis lung carcinoma had a hysteresis loop typical for a weak soft ferromagnet in contrast to surrounding tissues, which were diamagnetic. Combined action of constant magnetic field and radio frequency induced moderate hyperthermia induced both antitumor and antimetastatic effects greater than conventional DOX alone.
Nano-immunoassay with improved performance for detection of cancer biomarkers
W3 Star-forming Region Nano-immunoassay utilizing surface-enhanced Raman scattering (SERS) effect is a promising analytical technique for early detection of cancer. In its current standing the assay is capable of discriminating samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility will extend practical applications of the SERS-based detection platforms to wider range of problems. In this report, we discuss several strategies designed to improve performance of the SERS-based detection system. We demonstrate that reproducibility of the platform is enhanced by using atomically smooth mica surface as a template for preparation of capture surface in SERS sandwich immunoassay. Furthermore, assay's stability and sensitivity can be further improved by using either polymer or graphene monolayer as a thin protective layer applied on top of the assay addresses. The protective layer renders signal to be more stable against photo-induced damage and carbonaceous contamination. From the Clinical Editor This study discusses strategies to improve the performance of nano-immunoassays utilizing surface-enhanced Raman scattering (SERS) effect , an analytical technique with high sensitivity that holds a great promise for early cancer biomarker detection.
Curcumin-encapsulated nanoparticles as innovative antimicrobial and wound healing agent
W3 Star-forming Region Burn wounds are often complicated by bacterial infection, contributing to morbidity and mortality. Agents commonly used to treat burn wound infection are limited by toxicity, incomplete microbial coverage, inadequate penetration, and rising resistance. Curcumin is a naturally derived substance with innate antimicrobial and wound healing properties. Acting by multiple mechanisms, curcumin is less likely than current antibiotics to select for resistant bacteria. Curcumin's poor aqueous solubility and rapid degradation profile hinder usage; nanoparticle encapsulation overcomes this pitfall and enables extended topical delivery of curcumin. In this study, we synthesized and characterized curcumin nanoparticles (curc-np), which inhibited in vitro growth of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa in dose-dependent fashion, and inhibited MRSA growth and enhanced wound healing in an in vivo murine wound model. Curc-np may represent a novel topical antimicrobial and wound healing adjuvant for infected burn wounds and other cutaneous injuries. From the Clinical Editor This team of authors created curcumin nanoparticles in an effort to overcome curcumin’s endogenously poor aqueous solubility and demonstrated in a murine model its antimicrobial and wound healing properties that also resulted in growth inhibition of MRSA and Pseudomonas aeruginosa.
Epigenetic Heterogeneity in HIV-1 Latency Establishment
W3 Star-forming Region Despite prolonged antiretroviral therapy, HIV-1 persists as transcriptionally inactive proviruses. The HIV-1 latency remains a principal obstacle in curing AIDS. It is important to understand mechanisms by which HIV-1 latency is established to make the latent reservoir smaller. We present a molecular characterization of distinct populations at an early phase of infection. We developed an original dual-color reporter virus to monitor LTR kinetics from establishment to maintenance stage. We found that there are two ways of latency establishment i.e., by immediate silencing and slow inactivation from active infection. Histone covalent modifications, particularly polycomb repressive complex 2 (PRC2)-mediated H3K27 trimethylation, appeared to dominate viral transcription at the early phase. PRC2 also contributes to time-dependent LTR dormancy in the chronic phase of the infection. Significant differences in sensitivity against several stimuli were observed between these two distinct populations. These results will expand our understanding of heterogeneous establishment of HIV-1 latency populations.
Molecularly imprinted polymer decorated nanoporous gold for highly selective and sensitive electrochemical sensors
W3 Star-forming Region Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric conductivity, while electrochemically synthesized MIP thin layer affords selectivity for specific recognition of MNZ molecules. For MNZ determination, the hybrid electrode shows two dynamic linear range of 5 × 10-11 to 1 × 10-9 mol L-1 and 1 × 10-9 to 1.4 × 10-6 mol L-1 with a remarkably low detection limit of 1.8 × 10-11 mol L-1 (S/N = 3). In addition, the sensor exhibits high binding affinity and selectivity towards MNZ with excellent reproducibility and stability. Finally, the reliability of MIP-NPGL for MNZ detection is proved in real fish tissue samples, demonstrating the potential for the proposed electrochemical sensors in monitoring drug and biological samples.

W3 Star-forming Region Most primary breast cancers express estrogen receptor ? and can be treated via endocrine therapy using anti-estrogens such as tamoxifen; however, acquired endocrine resistance is a critical issue. To identify tamoxifen response-related microRNAs (miRNAs) in breast cancer, MCF-7 cells infected with a lentiviral miRNA library were treated with 4-hydroxytamoxifen (OHT) or vehicle for 4 weeks, and the amounts of individual miRNA precursors that had integrated into the genome were evaluated by microarray. Compared to the vehicle-treated cells, 5 ‘dropout’ miRNAs, which were downregulated in OHT-treated cells, and 6 ‘retained’ miRNAs, which were upregulated in OHT-treated cells, were identified. Of the dropout miRNAs, we found that miR-574-3p expression was downregulated in clinical breast cancer tissues as compared with their paired adjacent tissues. In addition, anti-miR-574-3p reversed tamoxifen-mediated suppression of MCF-7 cell growth. Clathrin heavy chain (CLTC) was identified as a miR-574-3p target gene by in silico algorithms and luciferase reporter assay using the 3' untranslated region of CLTC mRNA. Interestingly, loss and gain of miR-574-3p function in MCF-7 cells causes CLTC to be upregulated and downregulated, respectively. These results suggest that functional screening mediated by miRNA libraries can provide new insights into the genes essential for tamoxifen response in breast cancer.

AKTUALITY | NEWS


Témata 2015
Ph.D. studium

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12.10.2014: Sondu brněnských a slovenských vědců vynese do stratosféry balón. Bude zkoumat, jak kosmické záření poškozuje DNA a jak ovlivňuje život ve vesmíru
informace
fotografie
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Journal of Metallomics and Nanotechnologie
laboratorní časopis
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Upozornění pro cestující týkající se výskytu horečky způsobené virem EBOLA - pdf

Prevalence of Papillomaviruses, Polyomaviruses, and Herpesviruses in Triple-Negative and Inflammatory Breast Tumors
MSc. Ana Maria Jimenez Jimenez
23. 01. 2015, od 15:00
pozvánka

Světový den AIDS, sumarizace a výzvy
Prof. Ing. René Kizek, Ph.D.
23. 01. 2015, 15:00 h
pozvánka

CECE 2014 - 11th International Interdisciplinary Meeting on Bioanalysis
Kristýna Šmerková
23. 01. 2015, od 13:00 h
pozvánka

Prionové proteiny a funkce mědi při neurodegenerativních onemocněních
Eliška Sedláčková
23. 01. 2014, od 12:00
pozvánka

Inovativní tištěný senzor pro detekci přítomnosti těžkých kovů ve vodném prostředí
Doc. RNDr. Vojtech Adam, Ph.D.
23. 01. 2015, 13:00 h
pozvánka

Akce

Současná kosmonautika a kosmické technologie-fotografie

Den AIDS - 1/12/2014

Sarkosin - natáčení

Journal of Metallomics and Nanotechnologies

NOVÉ PUBLIKACE

Mechanisms of Uptake and Interaction of Platinum Based Drugs in Eukaryotic Cells

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

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