ban ban logo

Je hanba, že se někdo dovede namáhat po mnoho let, aby se stal dobrým lékařem, obhájcem, učitelem nebo geometrem, a přitom není ochoten namáhat se příslušně dlouhý čas, aby se stal dobrým člověkem.

Galénos z Pergamu

Tisková zpráva
docx / www / www



Zprávy z laboratoře / Laboratory information

liposon liposon

Picture of the week

Pravidelný seminář (29 T)
Nanometer-sized electrodes have drawn considerable interest in recent years. One of the reasons is that with nanoelectrodes one can obtain a high rate of mass transport and study kinetics of fast heterogeneous electron transfer (ET) reactions. They can also be used for high-resolution chemical imaging of surfaces and interfaces and as microscopic chemical sensors. Metallic nanowires, carbon nanotubes, magnetic nanoparticles, and metal oxide nanowires have been employed to fabricate nanoelectrodes and platforms. In this review, applications of single electrodes, nanoelectrode arrays, and ensembles are briefly evaluated, with emphasis on biological analysis. Nanoelectrodes offer great advantages in numerous areas of biological investigations, particularly in single cells studies, fabrication of microchips, design of coordinated biosensors, and in addressable patterned electrodes. Consequently, nanoelectrodes have immense potential in the development of efficient, specific, sensitive, and intelligent sensors. In conjunction with the rapidly evolving, cost-effective fabrication and materials development approaches, these sensors can be used as direct, point-of-care clinical devices, enabling more personalized medical care. The development and application of nanodevices in biology and medicine will have enormous implications for society and human health.

World AIDS day 2015

Pravidelný seminář (29 T)
World AIDS Day is held on 1 December each year and is an opportunity for people worldwide to unite in the fight against HIV, show their support for people living with HIV and to commemorate people who have died. World AIDS Day was the first ever global health day and the first one was held in 1988. Around 100,000 are currently living with HIV in the

UK and globally an estimated 34 million people have HIV. More than 35 million people have died from the virus, making it one of the most destructive pandemics in history. Today, many scientific advances have been made in HIV treatment, there are laws to protect people living with HIV and we understand so much more about the condition. But despite this, people do not know the facts about how to protect themselves and others from HIV, and stigma and discrimination remain a reality for many people living with HIV. World AIDS Day is important as it reminds the public and Government that HIV has not gone away – there is still a vital need to raise money, increase awareness, fight prejudice and improve education

XI. Diagnostic, Predictive and Experimental Oncology Days

About Conference:
You are cordially invited to attend the 11th annual cancer research conference, which creates an interdisciplinary platform to exchange and disseminate knowledge, meet your colleagues and collaborate with Czech and international experts involved in research, diagnostics and treatment of malignant tumors.
Dates of the event: December 2-3, 2015
Venue: Hotel nH Collection Olomouc Congress
Legionarska 21
Olomouc 779 00, Czech Republic

Analysis of Cisplatinated DNA Using Magnetic Immunoseparation

The Study and Characterization of Primary Squamous Cell Carcinoma of the Head and Neck and Their Malignant Potential

Influence of Metallothioneins on Binding between Cisplatin and DNA in Cisplatin Resistant and Non-Resistant Neuroblastoma Cells

Urinary Proteomics as a Tool for Discrimination of the Positive Surgical Margin Status after Radical Prostatectomy

International Volunteer Day 2015

Pravidelný seminář (29 T) International Volunteer Day (IVD) - (December 5) is an international observance designated by the United Nations since 1985. It offers an opportunity for volunteer organizations and individual volunteers to make visible their contributions - at local, national and international levels - to the achievement of the Millennium Development Goals (MDGs). The International Volunteer Day is marked by many non-governmental organizations, including Red Cross, scouts and others. It is also marked and supported by United Nations Volunteers. Apart from mobilising thousands of volunteers every year, the United Nations Volunteers (UNV) programme works closely with partners and governments to establish national volunteer programmes to create structures that foster and sustain local volunteerism in countries. Through the Online Volunteering service volunteers can take action for sustainable human development by supporting the activities of development organizations over the Internet. The main focus of IVD 2014 is not only celebrate volunteerism in all its facets – but also pay special tribute to people’s participation in making a difference locally, nationally and globally. IVD 2014 highlights the contribution of volunteers in engaging people from the grass-roots in decision-making processes, ultimately creating space for participation that leads to: stronger governance, social cohesion, peace and sustainable development.[1]

Journal of Metallomics and Nanotechnologies

JMetNano (29 T) Třetí číslo právě vyšlo
Časopis Journal of Metallomics and Nanotechnologies vychází pouze elektronicky, čtvrtletně. Jeho obsahové zaměření je v oblastí nano-biochemie, nanotechonologie, biomedicína a nanomedicína. Časopis vychází bez regionálních mutací v českém, slovenském a anglickém jazyce. Vydavatel: Laboratoř metalomiky a nanotechnologií Mendelova univerzita v Brně, Zemědělská 1, 613 00, Brno, Česká republika http://web2.mendelu.cz/af_239_nanotech/J_Met_Nano/index.html

Zprávy z výzkumu/ Scientific Reports

Microfluidic assay-based optical measurement techniques for cell analysis: A review of recent progress
 Země 2.0. NASA objevila planetu nejpodobnější té naší Since the early 2000s, microfluidic cell culture systems have attracted significant attention as a promising alternative to conventional cell culture methods and the importance of designing an efficient detection system to analyze cell behavior on a chip in real time is raised. For this reason, various measurement techniques for microfluidic devices have been developed with the development of microfluidic assays for high-throughput screening and mimicking of in vivo conditions. In this review, we discuss optical measurement techniques for microfluidic assays. First of all, the recent development of fluorescence- and absorbance-based optical measurement systems is described. Next, advanced optical detection systems are introduced with respect to three emphases: 1) optimization for long-term, real-time, and in situ measurements; 2) performance improvements; and 3) multimodal analysis conjugations. Moreover, we explore presents future prospects for the establishment of optical detection systems following the development of complex, multi-dimensional microfluidic cell culture assays to mimic in vivo tissue, organ, and human systems.
A facile and pragmatic electrochemical biosensing strategy for ultrasensitive detection of DNA in real sample based on defective T junction induced transcription amplification
 Země 2.0. NASA objevila planetu nejpodobnější té naší A novel and pragmatic electrochemical sensing strategy was developed for ultrasensitive and specific detection of nucleic acids by combining with defective T junction induced transcription amplification (DTITA). The homogeneous recognition and specific binding of target DNA with a pair of designed probes formed a defective T junction, further triggered primer extension reaction and in vitro transcription amplification to produce numerous single-stranded RNA. These RNA products of DTITA could hybridized with the biotinylated detection probes and immobilized capture probes for enzyme-amplified electrochemical detection on the surface of the biosensor. The proposed isothermal DTITA strategy displayed remarkable signal amplification performance and reproducibility. The electrochemical DNA biosensor showed very high sensitivity for target DNA with a low detection limit of 0.4 fM (240 molecules of the synthetic DNA), and can directly detect target pathogenic gene of Group B Streptococci (GBS) from as low as 400 copies of genomic DNA. Moreover, the established biosensor was successfully verified for directly identifying GBS in clinical samples. This proposed strategy presented a simple and pragmatic platform toward ultrasensitive and handy nucleic acids detection, and would become a potential tool for general application in point-of-care setting.
Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection
W3 Star-forming Region A turn-on competitive immunoassay using a low-cost Blu-ray optomagnetic setup and two differently sized magnetic particles (micron-sized particles acting as capture particles and nano-sized particles acting as detection particles) is here presented. For Salmonella detection, a limit of detection of 8×104 CFU/mL is achieved within a total assay time of 3 h. The combination of a competitive strategy and an optomagnetic setup not only enables a turn-on read-out format, but also results in a sensitivity limit about a factor of 20 times lower than of volumetric magnetic stray field detection device based immunoassays. The improvement of sensitivity is enabled by the formation of immuno-magnetic aggregates providing steric hindrance protecting the interior binding sites from interaction with the magnetic nanoparticle labels. The formation of immuno-magnetic aggregates is confirmed by fluorescence microscopy. The system exhibits no visible cross-reaction with other common pathogenic bacteria, even at concentrations as high as 107 CFU/mL. Furthermore, we present results when using the setup for a qualitative and homogeneous biplex immunoassay of Escherichia coli and Salmonella typhimurium.
Development of double-generation gold nanoparticle chip-based dengue virus detection system combining fluorescence turn-on probes
W3 Star-forming Region A sensing platform, combined amino acid labeling kit and a double-generation gold nanoparticle (DG-AuNP) chip, was designed to prove the existence of weak but crucial binding between the DV (dengue virus) and its CLEC5A receptor. At first, we have designed a kit combining the novel fluorescence turn-on sensors for lysine, arginine and cysteine amino acids. Advantages of this kit are that emission on–off switching can increase the signal-to-noise ratio and the virus must be fluorescently labelled with sufficient numbers of fluorophores because the lysine, arginine and cysteine residues of viral proteins are labelled simultaneously. Consequently, this kit can be used to light-on single DV spot both in solution and in cell under fluorescence microscopy. Second, the labeling kit was used to examine the DV binding to the CLEC5A-coated DG-AuNP chip. Based on our study, the double-generation gold nanoparticle construction of chip can support more coating areas for receptor CLEC5A and then, support more binding opportunities for DV. Meanwhile, the grooves between nanohemispheres will provide the extra driving force for DV stacking. We try to give a proof that this sensing platform is very useful for detection of weak binding mechanism.
An electrochemical nanobiosensor for plasma miRNA-155, based on graphene oxide and gold nanorod, for early detection of breast cancer
W3 Star-forming Region Circulating miRNAs are emerging as novel reliable biomarkers for early detection of cancer diseases. Through combining the advantages of electrochemical methods and nanomaterials with the selectivity of the oligo-hybridization-based biosensors, a novel electrochemical nanobiosensor for plasma miR-155 detection have demonstrated here, based on thiolated probe-functionalized gold nanorods (GNRs) decorated on the graphene oxide (GO) sheet on the surface of the glassy carbon electrode (GCE). The reduction signals of a novel intercalating label Oracet Blue (OB), were measured by differential pulse voltammetry (DPV) method. The transmission electron microscope (TEM) imaging, UV–vis spectrophotometry, cyclic voltammetry (CV), field emission scanning electron microscope (FE-SEM) imaging and energy dispersive spectroscopy (EDS) were proved the right synthesis of the GNRs and correct assembly of the modified electrode. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 2.0 fM to 8.0 pM, and the detection limit was 0.6 fM. Furthermore, the nanobiosensor showed high Specificity, and was able to discriminate sharply between complementary target miRNA, single-, three-base mismatch, and non-complementary miRNA. Alongside the outstanding sensitivity and selectivity, this nanobiosensor had great storage ability, reproducibility, and showed a decent response in the real sample analysis with plasma. In conclusion, the proposed electrochemical nanobiosensor could clinically be used in the early detection of the breast cancer, by direct detection of the plasma miR-155 in real clinical samples, without a need for sample preparation, RNA extraction and/or amplification.
Virus-based assay for antigen detection using infective growth as signal transduction mechanism
International Women’s Day Viruses have the ability to infect and thereby confer new phenotypes on host cells. E. coli, for example, if infected by viruses containing antibiotic resistance genes, can benefit by surviving in the presence of the corresponding antibiotics to grow into colonies observable by the naked eye. Using this concept as a signal transduction mechanism for our immunoassay, we have engineered ampicillin resistant virions to display a dimer of the z domain from Protein A. This zz-domain selectively binds to the conserved heavy domain of IgG across various species. As commercially available antibodies are in no short supply, this engineered virion can be used modularly with existing antibodies for converting the presence of target antigen into a visually detectable colony forming unit. H ere we demonstrate that this scheme for zz-phage transfection and selective growth of infected E. coli can facilitate sub-nanomolar detection limits for target antigen. Moreover, this phage infectivity assay works over a range of concentrations competitive with existing ELISA techniques. Because this system is derived from self-regenerating components (i.e., virus and bacteria) and furthermore obviates the need for chromogenic substrates or spectroscopic equipment, we find it particularly suitable for use in regions where cost effective detection is a necessity.
Rapid detection of multiple foodborne pathogens using a nanoparticle-functionalized multi-junction biosensor
International Women’s Day Real-time identification of multiple bacterial pathogens in food is urgently needed to ensure food safety. Although rapid and sensitive detection methods offering simplicity, accuracy, and multiplexity are highly desirable for industrial food applications, the development of a biosensor that meets all criteria remains a challenge. In this study, a single walled carbon nanotube- (SWCNT) based multi-junction sensor was designed for potential multiplexed detection of foodborne pathogens. Gold tungsten wires (O: 50 µm) coated with polyethylenimine (PEI) and SWCNTs were aligned to form a 2×2 junction array, functionalized with streptavidin and biotinylated antibodies specific for Escherichia coli K-12 and Staphylococcus aureus. Electric current (I) measurements in response to target binding events in pure serial diluted samples of E. coli and S. aureus at each junction within the 2×2 array were monitored to create calibration curves. An inverse correlation between I signals and bacterial concentrations was observed. Changes in I (?I) were also calculated to reduce background noise and emphasize the SWCNT-based sensor’s response to the biorecognition reactions between antibody and antigens. A linear regression was observed for both the E. coli and S. aureus functionalized array sensors, R2=0.978 and R2=0.992, in range of 102–105 CFU/mL. The calibration curves were used to evaluate the sensor’s multiplexing capabilities to detect E. coli and S. aureus in 10 µL and 100 µL batch microbial cocktail samples.Isignal responses exhibited similar measurement trends indicating that the developed SWCNT-based multi-junction biosensor has potential for sensitive, simple, and multiplexed applications.
Lable-free quadruple signal amplification strategy for sensitive electrochemical p53 gene biosensing
W3 Star-forming Region A versatile label-free quadruple signal amplification biosensing platform for p53 gene (target DNA) detection was proposed. The chitosan–graphene (CS–GR) modified electrode with excellent electron transfer ability could provide a large specific surface for high levels of AuNPs-DNA attachment. The large amount of AuNPs could immobilize more capture probes and enhance the electrochemical signal with the excellent electrocatalytic activity. Furthermore, with the assist of N.BstNB I (the nicking endonuclease), target DNA could be reused and more G-quadruplex-hemin DNAzyme could be formed, allowing significant signal amplification in the presence of H2O2. Such strategy can enhance the oxidation–reduction reaction of adsorbed methylene blue (MB) and efficiently improve the sensitivity of the proposed biosensor. The morphologies of materials and the stepwise biosensor were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). Differential pulse voltammetry (DPV) signals of MB provided quantitative measures of the concentrations of target DNA, with a linear calibration range of 1.0×10-15–1.0×10-9 M and a detection limit of 3.0×10-16 M. Moreover, the resulting biosensor also exhibited good specificity, acceptable reproducibility and stability, indicating that the present strategy was promising for broad potential application in clinic assay.
Quantification of cell viability and rapid screening anti-cancer drug utilizing nanomechanical fluctuation
W3 Star-forming Region Cancer is a serious threat to human health. Although numerous anti-cancer drugs are available clinically, many have shown toxic side effects due to poor tumor-selectivity, and reduced effectiveness due to cancers rapid development of resistance to treatment. The development of new highly efficient and practical methods to quantify cell viability and its change under drug treatment is thus of significant importance in both understanding of anti-cancer mechanism and anti-cancer drug screening. Here, we present an approach of utilizing a nanomechanical fluctuation based highly sensitive microcantilever sensor, which is capable of characterizing the viability of cells and quantitatively screening (within tens of minutes) their responses to a drug with the obvious advantages of a rapid, label-free, quantitative, noninvasive, real-time and in-situ assay. The microcantilever sensor operated in fluctuation mode was used in evaluating the paclitaxel effectiveness on breast cancer cell line MCF-7. This study demonstrated that the nanomechanical fluctuations of the microcantilever sensor are sensitive enough to detect the dynamic variation in cellular force which is provided by the cytoskeleton, using cell metabolism as its energy source, and the dynamic instability of microtubules plays an important role in the generation of the force. We propose that cell viability consists of two parts: biological viability and mechanical viability. Our experimental results suggest that paclitaxel has little effect on biological viability, but has a significant effect on mechanical viability. This new method provides a new concept and strategy for the evaluation of cell viability and the screening of anti-cancer drugs.
Sensitive and selective determination of GSH based on the ECL quenching of Ru(II) 1,10-phenanthroline-5,6-dione complex
W3 Star-forming Region Electrochemiluminescence (ECL) material Ru-dpq (Ru(bpy)2dpq2+, dpq=1,10-phenanthroline-5,6-dione; bpy=2,2'-bipyridine) is found to be produced strong and stable anodic ECL signal, which could be quenched by reduced glutathione (GSH) and exhibits high sensitivity and selectivity simultaneously. According to the mass spectra of Ru-SG (product of Ru-dpq reacted with GSH), and single crystal structure of the final oxidized product Ru-dcbpy ((Ru(bpy)2dcbpy2+, dcbpy=3,3-dicarboxy-2,2-bipyridine), we propose a new interacted mechanism between Ru-dpq and GSH. A good linear relation is estimated to be from 0.1 pM to 50 µM in the presence of calcium ion and the detection limit is as low as 0.087 pM (with the signal-to-noise ratio of 3). The relative standard deviation is 2.3% (for three repeated measurements). Furthermore, the ECL signal of Ru-dpq under a constant potential (1.2 V) is extremely stable and the intensity could be maintained over 600 s, which promotes us to determine the concentration of GSH via chronoamperometry.
A novel label-free electrochemical miRNA biosensor using methylene blue as redox indicator: application to breast cancer biomarker miRNA-21
W3 Star-forming Region Small noncoding microRNAs (miRNAs) have emerged as ideal noninvasive biomarkers for early-phase cancer detection. In this report, a label-free and simple electrochemical miRNA biosensor is developed based on employing methylene blue (MB) as a redox indicator. The successfully immobilization of the single strand DNA (ss-DNA) probe and hybridization with the target miRNA sequence were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods. Differential pulse voltammetry (DPV) technique was used to record the oxidation peak current of MB under optimal condition and an increase in the peak current was observed after hybridization. By employing this strategy, miRNA can detect in a range from 0.1 to 500.0 pM with a relatively low detection limit of 84.3 fM. The electrochemical response of MB on ss-DNA and duplex of miRNA/DNA was characterized by CV and chronocoulometry method. The linear relation between the redox peak currents (Ip) and scan rate (?) indicates that the electron transfer (ET) between MB and the electrode surface was mediated by the miRNA/DNA ?-stacked duplex. The value of surface coverage (?) was calculated that indicated increase amount of MB on the surface of modified electrode after hybridization event and revealed the adsorption of MB at modified electrode is monolayer. Also, the electron transfer rate constants (ks) of MB were estimated. The results of kinetic analysis were confirmed by chronocoulometry method. The discrimination ability of miRNA biosensor even against a noncomplementary target was also studied. Consequently, this strategy will be valuable for sensitive, selective and label-free detection of miRNA.
Paper-based chemical and biological sensors: Engineering aspects
W3 Star-forming Region Remarkable efforts have been dedicated to paper-based chemosensors and biosensors over the last few years, mainly driven by the promise of reaching the best trade-off between performance, affordability and simplicity. Because of the low-cost and rapid prototyping of these sensors, recent research has been focused on providing affordable diagnostic devices to the developing world. The recent progress in sensitivity, multi-functionality and integration of microfluidic paper-based analytical devices (µPADs), increasingly suggests that this technology is not only attractive in resource-limited environments but it also represents a serious challenger to silicon, glass and polymer-based biosensors. This review discusses the design, chemistry and engineering aspects of these developments, with a focus on the past few years. .W3 Star-forming Region
Sensitive and rapid detection of pathogenic bacteria in small volumes using impedance spectroscopy technique
W3 Star-forming Region We illustrate a novel impedance immunosensor which rapidly and sensitively detects typhoid-causing infectious bacteria Salmonella enterica serovar (Salmonella typhi) in 10 µL of sample volume. The bacteria are tagged with gold nanoparticles (Au NPs) via high-affinity antigen-antibody interactions for enhanced signal amplification and selectivity. The cell-particle bioconjugates are then subjected to alternating current (AC) electric fields applied through interdigitated microelectrodes. The immunosensor performance is optimized with respect to electric field frequency, cell concentration, incubation times and the type of blocking agent to achieve a low limit of detection (LOD) of 100 CFU/mL. The approach is extendable to a wide spectrum of clinical diseases and offers an efficient and cost-effective solution for point-of-care diagnosis.
A virus-based nanoplasmonic structure as a surface-enhanced Raman biosensor
W3 Star-forming Region Fabrication of nanoscale structures with localized surface plasmons allows for substantial increase in sensitivity of chem/bio sensors. The main challenge for realizing complex nanoplasmonic structures in solution is the high level of precision required at the nanoscale to position metal nanoparticles in 3D. In this study, we report a virus-like particle (VLP) for building a 3D plasmonic nanostructure in solution in which gold nanoparticles are precisely positioned on the VLP by directed self-assembly techniques. These structures allow for concentration of electromagnetic fields in the desired locations between the gold nanoparticles or “hot spots”. We measure the efficiency of the optical field spatial concentration for the first time, which results in a ten-fold enhancement of the capsid Raman peaks. Our experimental results agree with our 3D finite element simulations. Furthermore, we demonstrate as a proof-of-principle that the plasmonic nanostructures can be utilized in DNA detection down to 0.25 ng/µl (lowest concentration tested), while the protein peaks from the interior of the nanoplasmonic structures, potentially, can serve as an internal tracer for the biosensors.
Quantitative determination of uric acid using CdTe nanoparticles as fluorescence probes
W3 Star-forming Region A convenient enzymatic optical method for uric acid detection was developed based on the fluorescence quenching of ligand-capped CdTe nanoparticles by H2O2 which was generated from the enzymatic reaction of uric acid. The interactions between the CdTe nanoparticles capped with different ligands (glutathione, 3-mercaptopropionic acid, and thioglycerol) and H2O2 were investigated. The fluorescence quenching studies of GSH-capped CdTe nanoparticles demonstrated an excellent sensitivity to H2O2. The effects of uric acid, uricase and H2O2 on the fluorescence intensity of CdTe nanoparticles were also explored. The detection conditions, reaction time, pH value, incubation period and the concentration of uricase and uric acid were optimized. The detection limit of uric acid was found to be 0.10 µM and the linear range was 0.22–6 µM under the optimized experimental conditions. These results typify that CdTe nanoparticles could be used as a fluorescent probe for uric acid detection.
Sensitive and rapid detection of staphylococcus aureus in milk via cell binding domain of lysin
W3 Star-forming Region Staphylococcus aureus (S. aureus) is an important food-borne pathogen in dairy products contaminated through raw ingredients or improper food handling. Rapid detection of S. aureus with high sensitivity is of significance for food quality and safety. In this study, a new method was developed for detecting S. aureus in milk by coupling immunomagnetic separation with enzyme linked cell wall binding domain (CBD) of lysin plyV12, which can bind to S. aureus with high affinity. There are millions of binding sites present on the cell surface of S. aureus for the CBD attachment, which greatly improves the detection sensitivity. The method has the overall testing time of only 1.5 h with the detection limit of 4×103 CFU/mL in spiked milk. Because it is simple, rapid and sensitive, this method could be used for the detection of S. aureus in various food samples.
Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein
W3 Star-forming Region An Electrochemical Lateral Flow Immunosensor (ELFI) is developed combining screen-printed gold electrodes (SPGE) enabling quantification together with the convenience of a lateral flow test strip. A cellulose glassy fiber paper conjugate pad retains the marker immunoelectroactive nanobeads which will bind to the target analyte of interest. The specific immunorecognition event continues to occur along the lateral flow bed until reaching the SPGE-capture antibodies at the end of the cellulosic lateral flow strip. The rationale of the immunoassay consists in the analyte antigen NS1 protein being captured selectively and specifically by the dengue NS1 antibody conjugated onto the immunonanobeads thus forming an immunocomplex. With the aid of a running buffer, the immunocomplexes flow and reach the immuno-conjugated electrode surface and form specific sandwich-type detection due to specific, molecular recognition, while unbound beads move along past the electrodes. The successful sandwich immunocomplex formation is then recorded electrochemically. Specific detection of NS1 is translated into an electrochemical signal contributed by a redox label present on the bead-immobilized detection dengue NS1 antibody while a proportional increase of faradic current is observed with increase in analyte NS1 protein concentration. The first generation ELFI prototype is simply assembled in a cassette and successfully demonstrates wide linear range over a concentration range of 1–25 ng/mL with an ultrasensitive detection limit of 0.5 ng/mL for the qualitative and quantitative detection of analyte dengue NS1 protein.


Světový den AIDS – World AIDS day 2015
René Kizek, Petr Michálek, Natalia Cernei, Ana Maria Jimenez- Jimenez
01. 12. 2015, od 10:00

Analysis of Cisplatinated DNA Using Magnetic Immunoseparation
XI. Diagnostic, Predictive and Experimental Oncology Days

The Study and Characterization of Primary Squamous Cell Carcinoma of the Head and Neck and Their Malignant Potential
XI. Diagnostic, Predictive and Experimental Oncology Days

Influence of Metallothioneins on Binding between Cisplatin and DNA in Cisplatin Resistant and Non-Resistant Neuroblastoma Cells
XI. Diagnostic, Predictive and Experimental Oncology Days

Urinary Proteomics as a Tool for Discrimination of the Positive Surgical Margin Status after Radical Prostatectomy
XI. Diagnostic, Predictive and Experimental Oncology Days

Ebola response roadmap - Situation report XII
Ing. Bc. Petr Michálek
04. 12. 2015, 12:00 h

Izolace MT a histologie orgánů kuřat vystavených působení Zn-chelátů
Mgr. et Bc. Markéta Komínková
4. 12. 2015, 12:00 h

Hidden relationships between metalloproteins unveiled by structural comparison of their metal sites- review
RNDr. Ondřej Zítka, Ph.D.
4. 12. 2015, 15:00 h


XI. Diagnostic, Predictive and Experimental Oncology Days
2.12.2015,Olomouc www

Cena Karla Štulíka 2016
4.2.2016, Ústí na Labem www

Living Planet Symposium 2016
9.5.2016, Prague www

Journal of Metallomics and Nanotechnologies


Phage Capsid for Efficient Delivery of Cytotoxic Drugs

Zoonotické šíření ebola virů v rovníkové Africe a jejich vliv na dramatických úbytek počtů goril a šimpanzů

Zemědělská 1/1665
613 00 Brno
Budova D
Tel.: +420 545 133 350
Fax.: +420 545 212 044