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Charles Farrar Browne

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mendel
mendel

2014

Zprávy z laboratoře / Laboratory information

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Pravidelný seminář (29 T) Laboratory of synthesis – new heavy metal complexes for antimicrobial, antifungal and anticancer potential effects

NEW PROJECTS in the LAB

Pravidelný seminář (29 T) Nové dva evropské projekty vstupují do naší laboratoře. ID 120 Green Learning Network http://www.greenlearningnetwork.eu/ a projekt ID 134 Young Innovation brokers for Visegrad countries http://visegradfund.org/. Oba projekty jsou primárně zaměřeny na networking mezi evropskými státy.

SEMINAR

Pravidelný seminář (29 T)

RESEARCH IN LAB

From Na+/K+-ATPase and cardiac glycosides to cytotoxicity and cancer treatment
Pravidelný seminář (29 T)
The cardiac glycosides (CGs, also referred to as cardiac steroid glycosides) are a diverse family of naturally derived compounds, C23 or C24 steroidal glycosides that have been found in many angiosperms. The most important CG-containing plant families are Apocynaceae, incl. Asclepiadaceae (Adenium [1], Cerbera [2], Cryptostegia [3], Nerium [4, 5], Parepigynum [6, 7], Periploca [8-10], Strophanthus [11-15], Thevetia [16-22]), Brassicaceae (Erysimum [23-29], and Lepidium [30]), Celastraceae (Euonymus [31, 32], and Lophopetalum [33]), Convallariaceae (Convallaria [34-45]), Crassulaceae (Cotyledon [46], and Tylecodon [47, 48]), Hyacinthaceae (Schizobasis [49], and Urginea [50, 51]), Fabaceae (Coronilla [52, 53]), Malvaceae (Corchorus [54-56], and Mansonia [57, 58]), Moraceae (Antiaris [59-61], Castilla [62], Maquira [63-66], and Naucleopsis [65]), Ranunculaceae (Adonis [67-71], Eranthis [72], and Helleborus [73]), Scrophulariaceae s.s. (Digitalis [13, 74-91]), and Solanaceae (Nierembergia [92]).

Již před 10 lety se Česká republika spolu s dalšími devíti evropskými zeměmi staly členy Evropské unie. Ačkoliv si Česká republika oficiálně připomene svůj vstup do Evropské unie 1. května, Úřad vlády ČR ve spolupráci se Zastoupením Evropské komise v ČR a dalšími partnery přichystaly řadu akcí, které budou probíhat po celé jaro. Europe Day is observed annually on May 05, 2014. There are two separate designations of Europe Day: The Council of Europe's day was established in 1949, while the European Union's (EU) day celebrates the day the EU's predecessor was proposed in 1950. For the EU, the day is also known as Schuman Day, comme morating the historical declaration by French foreign minister Robert Schuman.

Journal of Metallomics and Nanotechnologies

První číslo vychází v průběhu května 2014 Časopis Journal of Metallomics and Nanotechnologies vychází pouze elektronicky, čtvrtletně. Jeho obsahové zaměření je v oblastí nano-biochemie, nanotechonologie, biomedicína a nanomedicína. Časopis vychází bez regionálních mutací v českém, slovenském a anglickém jazyce. Vydavatel: Laboratoř metalomiky a nanotechnologií Mendelova univerzita v Brně, Zemědělská 1, 613 00, Brno, Česká republika http://web2.mendelu.cz/af_239_nanotech/J_Met_Nano/index.html

Zprávy z výzkumu/ Scientific Reports



Testing Electric Propulsion
X-rays From A Young Supernova Remnant On Aug. 19, National Aviation Day, a lot of people are reflecting on how far aviation has come in the last century. Could this be the future – a plane with many electric motors that can hover like a helicopter and fly like a plane, and that could revolutionize air travel? Engineers at NASA's Langley Research Center in Hampton, Va., are studying the concept with models such as the unmanned aerial system GL-10 Greased Lightning. The GL-10, which has a 10-foot wingspan, recently flew successfully while tethered. Free-flight tests are planned in the fall of 2014. This research has helped lead to NASA Aeronautics Research Mission Directorate efforts to better understand the potential of electric propulsion across all types, sizes and missions for aviation.
Retreat of Yakutat Glacier
W3 Star-forming Region Located in the Brabazon Range of southeastern Alaska, Yakutat Glacier is one of the fastest retreating glaciers in the world. It is the primary outlet for the 810-square kilometer (310-square mile) Yakutat ice field, which drains into Harlequin Lake and, ultimately, the Gulf of Alaska. The Operational Land Imager on the Landsat 8 satellite captured this image of the glacier and lake on Aug. 13, 2013. Snow and ice appear white and forests are green. The brown streaks on the glaciers are lateral and medial moraines. Over the past 26 years, the glacier’s terminus has retreated more than 5 kilometers (3 miles). What is causing the rapid retreat? University of Alaska glaciologist Martin Truffer and colleagues pointed to a number of factors in their 2013 study published in the Journal of Glaciology. The chief cause is the long-term contraction of the Yakutat Ice Field, which has been shrinking since the height of the Little Ice Age. Once part of a much larger ice field, Yakutat has been contracting for hundreds of years. As other nearby glaciers retreated, Yakutat ice field was cut off from higher-elevation areas that once supplied a steady flow of ice from the north. With that flow cut off, there simply is not enough snow falling over the low-elevation Yakutat ice field to prevent it from retreating. Beyond this natural change, human-caused global warming has hastened the speed of the retreat. Between 1948–2000, mean annual temperatures in Yakutat increased by 1.38° Celsius (2.48° Fahrenheit). Between 2000 and 2010, they rose by another 0.48°C (0.86°F). The warmer temperatures encourage melting and sublimation at all ice surfaces exposed to the air. In the past few years, the breakdown of a long, floating ice tongue has triggered especially dramatic changes in the terminus of Yakutat glacier. For many years, Yakutat’s two main tributaries merged and formed a 5-kilometer (3-mile) calving face that extended far into Harlequin Lake. In the past decade, satellites observed a rapid terminus retreat and the breakup of the ice tongue in 2010. As a result, the calving front divided into two sections, with one running east-west and another running north-south.
Back Shell Tile Panels Installed on NASA's Orion Spacecraft
Inside the Operations and Checkout Building high bay at NASA's Kennedy Space Center in Florida, technicians dressed in clean-room suits have installed a back shell tile panel onto the Orion crew module and are checking the fit next to the middle back shell tile panel. Preparations are underway for Exploration Flight Test-1, or EFT-1. Orion is the exploration spacecraft designed to carry astronauts to destinations not yet explored by humans, including an asteroid and Mars. It will have emergency abort capability, sustain the crew during space travel and provide safe re-entry from deep space return velocities. The first unpiloted test flight of the Orion is scheduled to launch later this year atop a Delta IV rocket from Cape Canaveral Air Force Station in Florida to an altitude of 3,600 miles above the Earth's surface.
Multifunctional Enveloped Mesoporous Silica Nanoparticles for Subcellular Co-delivery of Drug and Therapeutic Peptide
Spacewalk ends early A multifunctional enveloped nanodevice based on mesoporous silica nanoparticle (MSN) was delicately designed for subcellular co-delivery of drug and therapeutic peptide to tumor cells. Mesoporous silica MCM-41 nanoparticles were used as the core for loading antineoplastic drug topotecan (TPT). The surface of nanoparticles was decorated with mitochondria-targeted therapeutic agent (Tpep) containing triphenylphosphonium (TPP) and antibiotic peptide (KLAKLAK)2 via disulfide linkage, followed by coating with a charge reversal polyanion poly(ethylene glycol)-blocked-2,3-dimethylmaleic anhydride-modified poly(L-lysine) (PEG-PLL(DMA)) via electrostatic interaction. It was found that the outer shielding layer could be removed at acidic tumor microenvironment due to the degradation of DMA blocks and the cellular uptake was significantly enhanced by the formation of cationic nanoparticles. After endocytosis, due to the cleavage of disulfide bonds in the presence of intracellular glutathione (GSH), pharmacological agents (Tpep and TPT) could be released from the nanoparticles and subsequently induce specific damage of tumor cell mitochondria and nucleus respectively with remarkable synergistic antitumor effect.
Physics of Nanomechanical Spectrometry of Viruses
Spacewalk ends early There is an emerging need of nanotools able to quantify the mechanical properties of single biological entities. A promising approach is the measurement of the shifts of the resonant frequencies of ultrathin cantilevers induced by the adsorption of the studied biological systems. Here, we present a detailed theoretical analysis to calculate the resonance frequency shift induced by the mechanical stiffness of viral nanotubes. The model accounts for the high surface-to-volume ratio featured by single biological entities, the shape anisotropy and the interfacial adhesion. The model is applied to the case in which tobacco mosaic virus is randomly delivered to a silicon nitride cantilever. The theoretical framework opens the door to a novel paradigm for biological spectrometry as well as for measuring the Young's modulus of biological systems with minimal strains. The two-orbit, four-hour flight test will help engineers evaluate the systems critical to crew safety including the heat shield, parachute system and launch abort system. Spacewalk ends early
Spatiotemporal Dynamics of Dissemination of Non-Pandemic HIV-1 Subtype B Clades in the Caribbean Region
Spacewalk ends early The Human immunodeficiency virus type-1 (HIV-1) epidemic in the Caribbean region is mostly driven by subtype B; but information about the pattern of viral spread in this geographic region is scarce and different studies point to quite divergent models of viral dissemination. In this study, we reconstructed the spatiotemporal and population dynamics of the HIV-1 subtype B epidemic in the Caribbean. A total of 1,806 HIV-1 subtype B pol sequences collected from 17 different Caribbean islands between 1996 and 2011 were analyzed together with sequences from the United States (n = 525) and France (n = 340) included as control. Maximum Likelihood phylogenetic analyses revealed that HIV-1 subtype B infections in the Caribbean are driven by dissemination of the pandemic clade (BPANDEMIC) responsible for most subtype B infections across the world, and older non-pandemic lineages (BCAR) characteristics of the Caribbean region. The non-pandemic BCAR strains account for >40% of HIV-1 infections in most Caribbean islands; with exception of Cuba and Puerto Rico. Bayesian phylogeographic analyses indicate that BCAR strains probably arose in the island of Hispaniola (Haiti/Dominican Republic) around the middle 1960s and were later disseminated to Trinidad and Tobago and to Jamaica between the late 1960s and the early 1970s. In the following years, the BCAR strains were also disseminated from Hispaniola and Trinidad and Tobago to other Lesser Antilles islands at multiple times. The BCAR clades circulating in Hispaniola, Jamaica and Trinidad and Tobago appear to have experienced an initial phase of exponential growth, with mean estimated growth rates of 0.35–0.45 year-1, followed by a more recent stabilization since the middle 1990s. These results demonstrate that non-pandemic subtype B lineages have been widely disseminated through the Caribbean since the late 1960s and account for an important fraction of current HIV-1 infections in the region.
The Vaginal Microbiota: What Have We Learned after a Decade of Molecular Characterization?
We conducted a systematic review of the Medline database (U.S. National Library of Medicine, National Institutes of Health, Bethesda, MD, U.S.A) to determine if consistent molecular vaginal microbiota (VMB) composition patterns can be discerned after a decade of molecular testing, and to evaluate demographic, behavioral and clinical determinants of VMB compositions. Studies were eligible when published between 1 January 2008 and 15 November 2013, and if at least one molecular technique (sequencing, PCR, DNA fingerprinting, or DNA hybridization) was used to characterize the VMB. Sixty three eligible studies were identified. These studies have now conclusively shown that lactobacilli-dominated VMB are associated with a healthy vaginal micro-environment and that bacterial vaginosis (BV) is best described as a polybacterial dysbiosis. The extent of dysbiosis correlates well with Nugent score and vaginal pH but not with the other Amsel criteria. Lactobacillus crispatus is more beneficial than L. iners. Longitudinal studies have shown that a L. crispatus-dominated VMB is more likely to shift to a L. iners-dominated or mixed lactobacilli VMB than to full dysbiosis. Data on VMB determinants are scarce and inconsistent, but dysbiosis is consistently associated with HIV, human papillomavirus (HPV), and Trichomonas vaginalis infection. In contrast, vaginal colonization with Candida spp. is more common in women with a lactobacilli-dominated VMB than in women with dysbiosis. Cervicovaginal mucosal immune responses to molecular VMB compositions have not yet been properly characterized. Molecular techniques have now become more affordable, and we make a case for incorporating them into larger epidemiological studies to address knowledge gaps in etiology and pathogenesis of dysbiosis, associations of different dysbiotic states with clinical outcomes, and to evaluate interventions aimed at restoring and maintaining a lactobacilli-dominated VMB.
Cationic Synthetic Peptides: Assessment of Their Antimicrobial Potency in Liquid Preserved Boar Semen
Spacewalk ends early Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination in liquid sperm preservation. Aminoglycosides are commonly supplemented to aid in the control of bacteria. As bacterial resistance is increasing worldwide, antimicrobial peptides (AMPs) received lively interest as alternatives to overcome multi-drug resistant bacteria. We investigated, whether synthetic cationic AMPs might be a suitable alternative for conventional antibiotics in liquid boar sperm preservation. The antibacterial activity of two cyclic AMPs (c-WWW, c-WFW) and a helical magainin II amide analog (MK5E) was studied in vitro against two Gram-positive and eleven Gram-negative bacteria. Isolates included ATCC reference strains, multi-resistant E. coli and bacteria cultured from boar semen. Using broth microdilution, minimum inhibitory concentrations were determined for all AMPs. All AMPs revealed activity towards the majority of bacteria but not against Proteus spp. (all AMPs) and Staphylococcus aureus ATCC 29213 (MK5E). We could also demonstrate that c-WWW and c-WFW were effective against bacterial growth in liquid preserved boar semen in situ, especially when combined with a small amount of gentamicin. Our results suggest that albeit not offering a complete alternative to traditional antibiotics, the use of AMPs offers a promising solution to decrease the use of conventional antibiotics and thereby limit the selection of multi-resistant strains.
Sialic Acid Metabolic Engineering: A Potential Strategy for the Neuroblastoma Therapy
Spacewalk ends early Human neuroblastoma SH-SY5Y cells were treated with synthetic Sia precursors N-propanoyl mannosamine (ManNProp) or N-pentanoyl mannosamine (ManNPent). Total and Polysialic acids (PolySia) were investigated by high performance liquid chromatography. Cell surface polySia were examined by flow-cytometry. Sia precursors treated cells were examined for the migration, invasion and sensitivity towards anticancer drugs and radiation treatment.
Results
Treatment of SH-SY5Y cells with ManNProp or ManNPent (referred as MSE) reduced their cell surface sialylation significantly. We found complete absence of polysialylation after treatment of SH-SY5Y cells with ManNPent. Loss of polysialylation results in a reduction of migration and invasion ability of these cells. Furthermore, radiation of Sia-engineered cells completely abolished their migration. In addition, MSE increases the cytotoxicity of anti-cancer drugs, such as 5-fluorouracil or cisplatin.
Conclusions
Metabolic Sia Engineering (MSE) of neuroblastoma cells using modified Sia precursors reduces their sialylation, metastatic potential and increases their sensitivity towards radiation or chemotherapeutics. Therefore, MSE may serve as an effective method to treat neuroblastoma
Arsenic Trioxide and Resveratrol Show Synergistic Anti-Leukemia Activity and Neutralized Cardiotoxicity
Spacewalk ends early Cardiotoxicity is an aggravating side effect of many clinical antineoplastic agents such as arsenic trioxide (As2O3), which is the first-line treatment for acute promyelocytic leukemia (APL). Clinically, drug combination strategies are widely applied for complex disease management. Here, an optimized, cardiac-friendly therapeutic strategy for APL was investigated using a combination of As2O3 and genistein or resveratrol. Potential combinations were explored with respect to their effects on mitochondrial membrane potential, reactive oxygen species, superoxide dismutase activity, autophagy, and apoptosis in both NB4 cells and neonatal rat left ventricular myocytes. All experiments consistently suggested that 5 µM resveratrol remarkably alleviates As2O3-induced cardiotoxicity. To achieve an equivalent effect, a 10-fold dosage of genistein was required, thus highlighting the dose advantage of resveratrol, as poor bioavailability is a common concern for its clinical application. Co-administration of resveratrol substantially amplified the anticancer effect of As2O3 in NB4 cells. Furthermore, resveratrol exacerbated oxidative stress, mitochondrial damage, and apoptosis, thereby reflecting its full range of synergism with As2O3. Addition of 5 µM resveratrol to the single drug formula of As2O3 also further increased the expression of LC3, a marker of cellular autophagy activity, indicating an involvement of autophagy-mediated tumor cell death in the synergistic action. Our results suggest a possible application of an As2O3 and resveratrol combination to treat APL in order to achieve superior therapeutics effects and prevent cardiotoxicity.
Recognition of Bungarus multicinctus Venom by a DNA Aptamer against ß-Bungarotoxin
Spacewalk ends early Antibody-based technology is the main method for diagnosis and treatment of snake bite envenoming currently. However, the development of an antibody, polyclonal or monoclonal, is a complicated and costly procedure. Aptamers are single stranded oligonucleotides that recognize specific targets such as proteins and have shown great potential over the years as diagnostic and therapeutic agents. In contrast to antibodies, aptamers can be selected in vitro without immunization of animals, and synthesized chemically with extreme accuracy, low cost and high degree of purity. In this study we firstly report on the identification of DNA aptamers that bind to ß-bungarotoxin (ß-BuTx), a neurotoxin from the venom of Bungarus multicinctus. A plate-SELEX method was used for the selection of ß-BuTx specific aptamers. After 10 rounds of selection, four aptamer candidates were obtained, with the dissociation constant ranged from 65.9 nM to 995 nM measured by fluorescence spectroscopy. Competitive binding assays using both the fluorescently labeled and unlabeled aptamers revealed that the four aptamers bound to the same binding site of ß-BuTx. The best binder, ßB-1, bound specifically to ß-BuTx, but not to BSA, casein or ?-Bungarotoxin. Moreover, electrophoretic mobility shift assay and enzyme-linked aptamer assay demonstrated that ßB-1 could discriminate B. multicinctus venom from other snake venoms tested. The results suggest that aptamer ßB-1 can serve as a useful tool for the design and development of drugs and diagnostic tests for ß-BuTx poisoning and B. multicinctus bites.
Mycobacterial and HIV Infections Up-Regulated Human Zinc Finger Protein 134
Spacewalk ends early Positive Regulator of HIV-1 LTR Activity and Viral Propagation
Concurrent occurrence of HIV and Tuberculosis (TB) infections influence the cellular environment of the host for synergistic existence. An elementary approach to understand such coalition at the molecular level is to understand the interactions of the host and the viral factors that subsequently effect viral replication. Long terminal repeats (LTR) of HIV genome serve as a template for binding trans-acting viral and cellular factors that regulate its transcriptional activity, thereby, deciding the fate of HIV pathogenesis, making it an ideal system to explore the interplay between HIV and the host.
Methodology/Principal Findings
In this study, using biotinylated full length HIV-1 LTR sequence as bait followed by MALDI analyses, we identified and further characterized human-Zinc-finger-protein-134 (hZNF-134) as a novel positive regulator of HIV-1 that promoted LTR-driven transcription and viral production. Over-expression of hZNF-134 promoted LTR driven luciferase activity and viral transcripts, resulting in increased virus production while siRNA mediated knockdown reduced both the viral transcripts and the viral titers, establishing hZNF-134 as a positive effector of HIV-1. HIV, Mycobacteria and HIV-TB co-infections increased hZNF-134 expressions in PBMCs, the impact being highest by mycobacteria. Corroborating these observations, primary TB patients (n = 22) recorded extraordinarily high transcript levels of hZNF-134 as compared to healthy controls (n = 16).
Conclusions/Significance
With these observations, it was concluded that hZNF-134, which promoted HIV-1 LTR activity acted as a positive regulator of HIV propagation in human host. High titers of hZNF-134 transcripts in TB patients suggest that up-regulation of such positive effectors of HIV-1 upon mycobacterial infection can be yet another mechanism by which mycobacteria assists HIV-1 propagation during HIV-TB co-infections. hZNF-134, an uncharacterized host protein, thus assumes a novel regulatory role during HIV-host interactions. Our study provides new insights into the emerging role of zinc finger proteins in HIV-1 pathogenesis.
Intranasal Delivery of Influenza rNP Adjuvanted with c-di-AMP Induces Strong Humoral and Cellular Immune Responses and Provides Protection against Virus Challenge/strong>
Spacewalk ends early There is a critical need for new influenza vaccines able to protect against constantly emerging divergent virus strains. This will be sustained by the induction of vigorous cellular responses and humoral immunity capable of acting at the portal of entry of this pathogen. In this study we evaluate the protective efficacy of intranasal vaccination with recombinant influenza nucleoprotein (rNP) co-administrated with bis-(3',5')-cyclic dimeric adenosine monophosphate (c-di-AMP) as adjuvant. Immunization of BALB/c mice with two doses of the formulation stimulates high titers of NP-specific IgG in serum and secretory IgA at mucosal sites. This formulation also promotes a strong Th1 response characterized by high secretion of INF-? and IL-2. The immune response elicited promotes efficient protection against virus challenge. These results suggest that c-di-AMP is a potent mucosal adjuvant which may significantly contribute towards the development of innovative mucosal vaccines against influenza.
Circulating miR-19a and miR-205 in Serum May Predict the Sensitivity of Luminal A Subtype of Breast Cancer Patients to Neoadjuvant Chemotherapy with Epirubicin Plus Paclitaxele
Spacewalk ends early The luminal A subtype of breast cancer has a good prognosis and is sensitive to endocrine therapy but is less sensitive to chemotherapy. It is necessary to identify biomarkers to predict chemosensitivity and avoid over-treatment. We hypothesized that miRNAs in the serum might be associated with chemosensitivity. Methods Sixty-eight breast cancer patients received neoadjuvant chemotherapy with epirubicin plus paclitaxel. The serum of the patients was collected before chemotherapy and stored at -80°C. The samples were classified into two groups in term of the chemosensitivity. We identified the differential expression patterns of miRNAs between the chemotherapy sensitive and resistant groups using microRNA profiling. Four miRNAs that were differentially expressed between the two groups were further validated in another 56 samples. We created a model fitting formula and a receiver operating characteristics (ROC) curve using logistic regression analysis to evaluate the prediction potency. Results We identified 8 miRNAs differentially expressed between the two groups: 6 miRNAs were up-regulated, and 2 miRNAs were down-regulated in the resistant group compared with the sensitive group. The expression of miR-19a and miR-205 were determined to have significant differences between the two groups (P<0.05). A predictive model of these two miRNAs was created by the logistic regression analysis. The probability of this model was 89.71%. Based on the ROC curve, the specificity was 75.00%, and the sensitivity was 81.25%. Conclusions The combination of miR-19a and miR-205 in the serum may predict the chemosensitivity of luminal A subtype of breast cancer to epirubicin plus paclitaxel neoadjuvant chemotherapy.
Is Thymidine Glycol Containing DNA a Substrate of E. coli DNA Mismatch Repair System?
Spacewalk ends early The DNA mismatch repair (MMR) system plays a crucial role in the prevention of replication errors and in the correction of some oxidative damages of DNA bases. In the present work the most abundant oxidized pyrimidine lesion, 5,6-dihydro-5,6-dihydroxythymidine (thymidine glycol, Tg) was tested for being recognized and processed by the E. coli MMR system, namely complex of MutS, MutL and MutH proteins. In a partially reconstituted MMR system with MutS-MutL-MutH proteins, G/Tg and A/Tg containing plasmids failed to provoke the incision of DNA. Tg residue in the 30-mer DNA duplex destabilized double helix due to stacking disruption with neighboring bases. However, such local structural changes are not important for E. coli MMR system to recognize this lesion. A lack of repair of Tg containing DNA could be due to a failure of MutS (a first acting protein of MMR system) to interact with modified DNA in a proper way. It was shown that Tg in DNA does not affect on ATPase activity of MutS. On the other hand, MutS binding affinities to DNA containing Tg in G/Tg and A/Tg pairs are lower than to DNA with a G/T mismatch and similar to canonical DNA. Peculiarities of MutS interaction with DNA was monitored by Förster resonance energy transfer (FRET) and fluorescence anisotropy. Binding of MutS to Tg containing DNAs did not result in the formation of characteristic DNA kink. Nevertheless, MutS homodimer orientation on Tg-DNA is similar to that in the case of G/T-DNA. In contrast to G/T-DNA, neither G/Tg- nor A/Tg-DNA was able to stimulate ADP release from MutS better than canonical DNA. Thus, Tg residue in DNA is unlikely to be recognized or processed by the E. coli MMR system. Probably, the MutS transformation to active “sliding clamp” conformation on Tg-DNA is problematic.

AKTUALITY | NEWS


Journal of Metallomics and Nanotechnologie
laboratorní časopis
- informace pro autory

Upozornění pro cestující týkající se výskytu horečky způsobené virem EBOLA - pdf

Vlastní technická a technologická příprava pro stratosferický let
Jan Zítka
27. 8. 2014, 15:00 h
pozvánka

Vývoj nových nástrojů pro rychlou správu experimentálních a provozních dat v systému
Prof. Ing. René Kizek, Ph.D.
úterý 28. 08. 2014, od 14:00 – 18:00 h
pozvánka

Využití nanotechnologií pro studim interakcí s nukleovou kyselinou
Martina Matoušková
pátek 29. 08. 2014, od 12:00
pozvánka

Metody pro studium interakce receptoru pro hemaglutinin
Petr Michálek
29. 08. 2014, od 12:00
pozvánka

Introduction of MALDI-TOF: A revolution in proteins detection
Miguel Ángel Merlos Rodrigo
29. 08. 2014, od 12:00
pozvánka

Aptamery, jejich funkce a využití v nanotechnologii
Monika Kremplová
29. 08. 2014, od 12:00
pozvánka

AFM meeting in Karlsruhe
Zbyněk Heger
29. 08. 2014, od 14:00
pozvánka

Optimalizace separace a detekce putrescinu pomocí HPLC s derivatizací ninhydrinem
Markéta Komínková
29. 08. 2014, od 15:00
pozvánka

Akce

Nanocon 2014
6. mezinárodní konference
5. - 7. listopadu 2014 - Hotel Voroněž I, Brno, Česká republika, EU
pozvánka

Journal of Metallomics and Nanotechnologies

Prototyp funkční vzorek

Způsob detekce virových hemaglutininů pomocí anorganických nanočástic ukotvených na chromatografickém papíru

Komplex biopolymerní látky s nanočásticemi selenu a antibiotickými léčivy s antibakteriálním účinkem

Informační systém pro vážení

NOVÉ PUBLIKACE

Monitoring of the Surface Modification of Nanoparticles by Electrochemical Measurements Using Scanning Electrochemical Microscope

The effect of silver ions and silver nanoparticles on Staphylococcus aureus

Expression Levels of Enzymes Metabolizing an Anticancer Drug Ellipticine Determined by Electromigration Assays Influence its Cytotoxicity to Cancer Cells - A Comparative Study

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